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Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis

Citation

Price, EP and Sarovich, DS and Webb, JR and Hall, CM and Jaramillo, SA and Sahl, JW and Kaestli, M and Mayo, M and Harrington, G and Baker, AL and Sidak-Loftis, LC and Settles, EW and Lummis, M and Schupp, JM and Gillece, JD and Tuanyok, A and Warner, J and Busch, JD and Keim, P and Currie, BJ and Wagner, DM, Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis, PLoS Neglected Tropical Diseases, 11, (9) Article e0005928. ISSN 1935-2727 (2017) [Refereed Article]


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Copyright 2017 Price et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pntd.0005928

Abstract

The bacterium Burkholderia ubonensis is commonly co-isolated from environmental specimens harbouring the melioidosis pathogen, Burkholderia pseudomallei. B. ubonensis has been reported in northern Australia and Thailand but not North America, suggesting similar geographic distribution to B. pseudomallei. Unlike most other Burkholderia cepacia complex (Bcc) species, B. ubonensis is considered non-pathogenic, although its virulence potential has not been tested. Antibiotic resistance in B. ubonensis, particularly towards drugs used to treat the most severe B. pseudomallei infections, has also been poorly characterised. This study examined the population biology of B. ubonensis, and includes the first reported isolates from the Caribbean. Phylogenomic analysis of 264 B. ubonensis genomes identified distinct clades that corresponded with geographic origin, similar to B. pseudomallei. A small proportion (4%) of strains lacked the 920kb chromosome III replicon, with discordance of presence/absence amongst genetically highly related strains, demonstrating that the third chromosome of B. ubonensis, like other Bcc species, probably encodes for a nonessential pC3 megaplasmid. Multilocus sequence typing using the B. pseudomallei scheme revealed that one-third of strains lack the "housekeeping" narK locus. In comparison, all strains could be genotyped using the Bcc scheme. Several strains possessed high-level meropenem resistance (≥32 μg/mL), a concern due to potential transmission of this phenotype to B. pseudomallei. In silico analysis uncovered a high degree of heterogeneity among the lipopolysaccharide O-antigen cluster loci, with at least 35 different variants identified. Finally, we show that Asian B. ubonensis isolate RF23-BP41 is avirulent in the BALB/c mouse model via a subcutaneous route of infection. Our results provide several new insights into the biology of this understudied species.

Item Details

Item Type:Refereed Article
Keywords:Burkholderia ubonensis, Burkholderia pseudomallei, phylogeographic, genomic
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Genetic Immunology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Agricultural and Veterinary Sciences
Author:Baker, AL (Dr Anthony Baker)
ID Code:125883
Year Published:2017
Deposited By:TIA - Research Institute
Deposited On:2018-05-14
Last Modified:2018-06-28
Downloads:5 View Download Statistics

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