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Altered expression of a malate-permeable anion channel, OsALMT4, disrupts mineral nutrition

Citation

Liu, J and Zhou, M and Delhaize, E and Ryan, PR, Altered expression of a malate-permeable anion channel, OsALMT4, disrupts mineral nutrition, Plant Physiology, 175, (4) pp. 1745-1759. ISSN 0032-0889 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 American Society of Plant Biologists. All Rights Reserved.

DOI: doi:10.1104/pp.17.01142

Abstract

Aluminum-activated malate transporters (ALMTs) form a family of anion channels in plants, but little is known about most of its members. This study examined the function of OsALMT4 from rice (Oryza sativa). We show that OsALMT4 is expressed in roots and shoots and that the OsALMT4 protein localizes to the plasma membrane. Transgenic rice lines overexpressing (OX) OsALMT4 released malate from the roots constitutively and had 2-fold higher malate concentrations in the xylem sap than nulls, indicating greater concentrations of malate in the apoplast. OX lines developed brown necrotic spots on the leaves that did not appear on nulls. These symptoms were not associated with altered concentrations of any mineral element in the leaves, although the OX lines had higher concentrations of Mn and B in their grain compared with nulls. While total leaf Mn concentrations were not different between the OX and null lines, Mn concentrations in the apoplast were greater in the OX plants. The OX lines also displayed increased expression of Mn transporters and were more sensitive to Mn toxicity than null plants. We showed that the growth of wild-type rice was unaffected by 100 M Mn in hydroponics but, when combined with 1 mM malate, this concentration inhibited growth. We conclude that increasing OsALMT4 expression affected malate efflux and compartmentation within the tissues, which increased Mn concentrations in the apoplast of leaves and induced the toxicity symptoms. This study reveals new links between malate transport and mineral nutrition.

Item Details

Item Type:Refereed Article
Keywords:Al tolerance, gene expression, anion channel, OsALMT4, mineral nutrition, rice
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
UTAS Author:Liu, J (Mrs Jie Liu)
UTAS Author:Zhou, M (Professor Meixue Zhou)
ID Code:125788
Year Published:2017
Web of Science® Times Cited:7
Deposited By:TIA - Research Institute
Deposited On:2018-05-05
Last Modified:2018-07-31
Downloads:0

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