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The ability to regulate voltage-gated K+-permeable channels in the mature root epidermis is essential for waterlogging tolerance in barley


Gill, MB and Zeng, F and Shabala, L and Bohm, J and Zhang, G and Zhou, M and Shabala, S, The ability to regulate voltage-gated K+-permeable channels in the mature root epidermis is essential for waterlogging tolerance in barley, Journal of Experimental Botany, 69, (3) pp. 667-680. ISSN 0022-0957 (2018) [Refereed Article]


Copyright Statement

© The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1093/jxb/erx429


Oxygen depletion under waterlogged conditions results in a compromised operation of H+-ATPase, with strong implications for membrane potential maintenance, cytosolic pH homeostasis, and transport of all nutrients across membranes. The above effects, however, are highly tissue specific and time dependent, and the causal link between hypoxia-induced changes to the cellís ionome and plant adaptive responses to hypoxia is not well established. This work aimed to fill this gap and investigate the effects of oxygen deprivation on K+ signalling and homeostasis in plants, and potential roles of GORK (depolarization-activated outward-rectifying potassium) channels in adaptation to oxygen-deprived conditions in barley. A significant K+ loss was observed in roots exposed to hypoxic conditions; this loss correlated with the cellís viability. Stress-induced K+ loss was stronger in the root apex immediately after stress onset, but became more pronounced in the root base as the stress progressed. The amount of K+ in shoots of plants grown in waterlogged soil correlated strongly with K+ flux under hypoxia measured in laboratory experiments. Hypoxia induced membrane depolarization; the severity of this depolarization was less pronounced in the tolerant group of cultivars. The expression of GORK was down-regulated by 1.5-fold in mature root but it was up-regulated by 10-fold in the apex after 48 h hypoxia stress. Taken together, our results suggest that the GORK channel plays a central role in K+ retention and signalling under hypoxia stress, and measuring hypoxia-induced K+ fluxes from the mature root zone may be used as a physiological marker to select waterlogging-tolerant varieties in breeding programmes.

Item Details

Item Type:Refereed Article
Keywords:GORK, H+-ATPase, H+-PPase, hypoxia, ionic homeostasis, potassium, signalling, viability staining, waterlogging.
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Gill, MB (Mr Muhammad Gill)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Bohm, J (Ms Jennifer Bohm)
UTAS Author:Zhou, M (Professor Meixue Zhou)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:125785
Year Published:2018
Web of Science® Times Cited:25
Deposited By:TIA - Research Institute
Deposited On:2018-05-05
Last Modified:2019-03-20
Downloads:70 View Download Statistics

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