eCite Digital Repository

Single cell RNA sequencing of stem cell-derived retinal ganglion cells

Citation

Daniszewski, M and Senabouth, A and Nguyen, QH and Crombie, DE and Lukowski, SW and Kulkarni, T and Sluch, VM and Jabbari, JS and Chamling, X and Zack, DJ and Pebay, A and Powell, JE and Hewitt, AW, Single cell RNA sequencing of stem cell-derived retinal ganglion cells, Scientific Data, 5 Article 180013. ISSN 2052-4463 (2018) [Refereed Article]


Preview		PDF
2Mb		  

Copyright Statement

© 2018 The Authors. © 2018 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ Metadata Licensed under Creative Commons Attribution CC0 1.0 Universal (CC0 1.0) https://creativecommons.org/publicdomain/zero/1.0/

DOI: doi:10.1038/sdata.2018.13

Abstract

We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature RGC phenotype.

Item Details

Item Type:Refereed Article
Keywords:data, single cell RNA, retinal ganglion cells, embryonic stem cell
Research Division:Medical and Health Sciences
Research Group:Ophthalmology and Optometry
Research Field:Ophthalmology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Hearing, Vision, Speech and Their Disorders
UTAS Author:Hewitt, AW (Professor Alex Hewitt)
ID Code:125738
Year Published:2018
Web of Science® Times Cited:6
Deposited By:Menzies Institute for Medical Research
Deposited On:2018-05-02
Last Modified:2019-01-17
Downloads:64 View Download Statistics

Repository Staff Only: item control page