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The pro-inflammatory effects of particulate matter on epithelial cells are associated with elemental composition

Citation

Shao, J and Wheeler, AJ and Chen, L and Strandberg, B and Hinwood, A and Johnston, FH and Zosky, GR, The pro-inflammatory effects of particulate matter on epithelial cells are associated with elemental composition, Chemosphere, 202 pp. 530-537. ISSN 0045-6535 (2018) [Refereed Article]

Copyright Statement

Copyright 2018 Elsevier Ltd.

DOI: doi:10.1016/j.chemosphere.2018.03.052

Abstract

Background: Adverse health effects of particulate matter (PM) vary with chemical composition; however, evidence regarding which elements are the most detrimental is limited. The roof space area provides an open and stable environment for outdoor PM to settle and deposit. Therefore, this study used roof space PM samples as a proxy of residential cumulative exposure to outdoor air pollution to investigate their pro-inflammatory effects on human lung cells and the contribution of the endotoxin and chemical content.

Methods: Roof space PM samples of 36 different homes were collected and analysed using standardised techniques. We evaluated cytotoxicity and cytokine production of BEAS-2B cells after PM exposure using MTS and ELISA, respectively. Principle component analysis (PCA) and linear regression analyses were employed to assess the associations between cytokine production and the PM components.

Results: PM caused significant time- and dose-dependent increases in cellular cytokine production (p < 0.05). PCA identified four factors that explained 68.33% of the variance in the chemical composition. An increase in Factor 1 (+Fe, +Al, +Mn) score and a decrease in Factor 2 (-Ca, +Pb, +PAH) score were associated with increased interleukin (IL)-6 (Factor 1; p = 0.010; Factor 2; p = 0.006) and IL-8 (Factor 1; p = 0.003; Factor 2; p = 0.020) production, however, only the association with Factor 1 was evident after correcting for endotoxin and particle size.

Conclusions: Our study provides novel insight into the positive associations between pro-inflammatory effects of roof space PM samples with Fe, Al and Mn levels.

Item Details

Item Type:Refereed Article
Keywords:air pollution, element, inflammation, particulate matter
Research Division:Environmental Sciences
Research Group:Environmental Science and Management
Research Field:Environmental Monitoring
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Environmental Health
UTAS Author:Shao, J (Miss Jingyi Shao)
UTAS Author:Wheeler, AJ (Dr Amanda Wheeler)
UTAS Author:Chen, L (Dr Ling Chen)
UTAS Author:Johnston, FH (Associate Professor Fay Johnston)
UTAS Author:Zosky, GR (Professor Graeme Zosky)
ID Code:125726
Year Published:2018
Web of Science® Times Cited:5
Deposited By:Menzies Institute for Medical Research
Deposited On:2018-05-02
Last Modified:2019-01-17
Downloads:0

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