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The pro-inflammatory effects of particulate matter on epithelial cells are associated with elemental composition
Shao, J and Wheeler, AJ and Chen, L and Strandberg, B and Hinwood, A and Johnston, FH and Zosky, GR, The pro-inflammatory effects of particulate matter on epithelial cells are associated with elemental composition, Chemosphere, 202 pp. 530-537. ISSN 0045-6535 (2018) [Refereed Article]
Copyright 2018 Elsevier Ltd.
Methods: Roof space PM samples of 36 different homes were collected and analysed using standardised techniques. We evaluated cytotoxicity and cytokine production of BEAS-2B cells after PM exposure using MTS and ELISA, respectively. Principle component analysis (PCA) and linear regression analyses were employed to assess the associations between cytokine production and the PM components.
Results: PM caused significant time- and dose-dependent increases in cellular cytokine production (p < 0.05). PCA identified four factors that explained 68.33% of the variance in the chemical composition. An increase in Factor 1 (+Fe, +Al, +Mn) score and a decrease in Factor 2 (-Ca, +Pb, +PAH) score were associated with increased interleukin (IL)-6 (Factor 1; p = 0.010; Factor 2; p = 0.006) and IL-8 (Factor 1; p = 0.003; Factor 2; p = 0.020) production, however, only the association with Factor 1 was evident after correcting for endotoxin and particle size.
Conclusions: Our study provides novel insight into the positive associations between pro-inflammatory effects of roof space PM samples with Fe, Al and Mn levels.
|Item Type:||Refereed Article|
|Keywords:||air pollution, element, inflammation, particulate matter|
|Research Division:||Environmental Sciences|
|Research Group:||Pollution and contamination|
|Research Field:||Pollution and contamination not elsewhere classified|
|Objective Group:||Public health (excl. specific population health)|
|Objective Field:||Public health (excl. specific population health) not elsewhere classified|
|UTAS Author:||Shao, J (Ms Jingyi Shao)|
|UTAS Author:||Wheeler, AJ (Dr Amanda Wheeler)|
|UTAS Author:||Chen, L (Dr Ling Chen)|
|UTAS Author:||Johnston, FH (Professor Fay Johnston)|
|UTAS Author:||Zosky, GR (Professor Graeme Zosky)|
|Web of Science® Times Cited:||16|
|Deposited By:||Menzies Institute for Medical Research|
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