Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography is introduced. Modulation optimization involved a systematic investigation of heat transfer, analyte retention, and migration velocity at a range of temperatures. Longitudinal on-column thermal modulation was realized using a set of alkylphenones and compared to a conventional valve-modulator employing sample loops. The thermal modulator showed a reduced modulation-induced pressure impact than valve modulation, resulting in reduced baseline perturbation by a factor of 6; yielding a 6-14-fold improvement in signal-to-noise. A red wine sample was analyzed to demonstrate the potential of the longitudinal on-column thermal modulator for separation of a complex sample. Discrete peaks in the second dimension using the thermal modulator were 30-55% narrower than with the valve modulator. The results shown herein demonstrate the benefits of an active focusing modulator, such as reduced detection limits and increased total peak capacity.

Item Type:	Refereed Article
Keywords:	chromatography, heat transfer, liquid chromatography
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Creese, ME (Dr Mari Creese)
UTAS Author:	Creese, MJ (Mr Matthew Creese)
UTAS Author:	Foley, JP (Professor Joe Foley)
UTAS Author:	Hilder, EF (Professor Emily Hilder)
UTAS Author:	Shellie, RA (Associate Professor Robert Shellie)
UTAS Author:	Breadmore, MC (Professor Michael Breadmore)
ID Code:	125358
Year Published:	2017
Web of Science® Times Cited:	14
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2018-04-13
Last Modified:	2018-07-27
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