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Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography

Citation

Creese, ME and Creese, MJ and Foley, JP and Cortes, HJ and Hilder, EF and Shellie, RA and Breadmore, MC, Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography, Analytical Chemistry, 89, (2) pp. 1123-1130. ISSN 0003-2700 (2017) [Refereed Article]


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DOI: doi:10.1021/acs.analchem.6b03279

Abstract

Longitudinal on-column thermal modulation for comprehensive two-dimensional liquid chromatography is introduced. Modulation optimization involved a systematic investigation of heat transfer, analyte retention, and migration velocity at a range of temperatures. Longitudinal on-column thermal modulation was realized using a set of alkylphenones and compared to a conventional valve-modulator employing sample loops. The thermal modulator showed a reduced modulation-induced pressure impact than valve modulation, resulting in reduced baseline perturbation by a factor of 6; yielding a 6-14-fold improvement in signal-to-noise. A red wine sample was analyzed to demonstrate the potential of the longitudinal on-column thermal modulator for separation of a complex sample. Discrete peaks in the second dimension using the thermal modulator were 30-55% narrower than with the valve modulator. The results shown herein demonstrate the benefits of an active focusing modulator, such as reduced detection limits and increased total peak capacity.

Item Details

Item Type:Refereed Article
Keywords:chromatography, heat transfer, liquid chromatography
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Creese, ME (Mrs Mari Creese)
Author:Creese, MJ (Mr Matthew Creese)
Author:Foley, JP (Professor Joe Foley)
Author:Hilder, EF (Professor Emily Hilder)
Author:Shellie, RA (Associate Professor Robert Shellie)
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:125358
Year Published:2017
Web of Science® Times Cited:4
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2018-04-13
Last Modified:2018-04-13
Downloads:0

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