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Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma-mass spectrometry


Bishop, DP and Blanes, L and Wilson, AB and Wilbanks, T and Killeen, K and Grimm, R and Wenzel, R and Major, D and Macka, M and Clarke, D and Schmid, R and Cole, N and Doble, PA, Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma-mass spectrometry, Journal of Chromatography A, 1497 pp. 64-69. ISSN 0021-9673 (2017) [Refereed Article]

Copyright Statement

© 2017 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.chroma.2017.03.025


The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300nLmin-1. The potential of the instrument to eliminate common nanoLC - ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2 of 0.9999, the peak area repeatability was 1.9% (n=7), and the detection limit was 14ngmL-1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.

Item Details

Item Type:Refereed Article
Keywords:NanoLC-ICP–MS, microfluidics, chip HPLC, cobalamin
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Macka, M (Professor Mirek Macka)
ID Code:125306
Year Published:2017
Web of Science® Times Cited:20
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2018-04-12
Last Modified:2018-06-22

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