The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300nLmin^-1. The potential of the instrument to eliminate common nanoLC - ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r² of 0.9999, the peak area repeatability was 1.9% (n=7), and the detection limit was 14ngmL^-1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.

Item Type:	Refereed Article
Keywords:	NanoLC-ICP–MS, microfluidics, chip HPLC, cobalamin
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
Author:	Macka, M (Professor Mirek Macka)
ID Code:	125306
Year Published:	2017
Web of Science® Times Cited:	6
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2018-04-12
Last Modified:	2018-06-22
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