eCite Digital Repository
Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection: a fast new approach to selective derivatization techniques
Citation
Pravadali-Cekic, S and Jones, A and Kazarian, AA and Paull, B and Soliven, A and Ritchie, H and Camenzuli, M and Leung, L and Dennis, GR and Shalliker, RA, Postcolumn derivatization of amino acids using reaction flow chromatography columns with fluorescence detection: a fast new approach to selective derivatization techniques, Journal of Liquid Chromatography and Related Technologies, 40, (2) pp. 83-92. ISSN 1082-6076 (2017) [Refereed Article]
Copyright Statement
© 2017 Taylor & Francis
DOI: doi:10.1080/10826076.2017.1285312
Abstract
Reaction flow (RF) chromatography with fluorescamine reagent and fluorescence detection (FLD) was used for the analysis of amino acids. The performance of RF chromatography was tested against several optimized conventional postcolumn derivatization (PCD) methods. RF columns achieved greater sensitivity compared to conventional PCD methods, without the need for reaction loops, which resulted in more efficient separations. The RF-PCD method also achieved limits of detection (LOD) from the low picomole to subnanomole range. The calibration data of the RF-PCD technique yielded R2 ≥ 0.99 and % relative standard deviation in peak areas ranging from 0.34% to 5%. Through reaction flow chromatography, multiplexed detection was also achieved allowing the monitoring and analysis of derivatized and nonderivatized flow streams simultaneously.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | amino acids, fluorescamine, fluorescence detection, multiplexed detection, postcolumn derivatization, reaction flow chromatography |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Kazarian, AA (Dr Tom Kazarian) |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 124819 |
Year Published: | 2017 |
Web of Science® Times Cited: | 7 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2018-03-13 |
Last Modified: | 2018-07-19 |
Downloads: | 0 |
Repository Staff Only: item control page