Reaction flow (RF) chromatography with fluorescamine reagent and fluorescence detection (FLD) was used for the analysis of amino acids. The performance of RF chromatography was tested against several optimized conventional postcolumn derivatization (PCD) methods. RF columns achieved greater sensitivity compared to conventional PCD methods, without the need for reaction loops, which resulted in more efficient separations. The RF-PCD method also achieved limits of detection (LOD) from the low picomole to subnanomole range. The calibration data of the RF-PCD technique yielded R² ≥ 0.99 and % relative standard deviation in peak areas ranging from 0.34% to 5%. Through reaction flow chromatography, multiplexed detection was also achieved allowing the monitoring and analysis of derivatized and nonderivatized flow streams simultaneously.

Item Type:	Refereed Article
Keywords:	amino acids, fluorescamine, fluorescence detection, multiplexed detection, postcolumn derivatization, reaction flow chromatography
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
UTAS Author:	Kazarian, AA (Dr Tom Kazarian)
UTAS Author:	Paull, B (Professor Brett Paull)
ID Code:	124819
Year Published:	2017
Web of Science® Times Cited:	6
Deposited By:	Austn Centre for Research in Separation Science
Deposited On:	2018-03-13
Last Modified:	2018-07-19
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