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Effect of 1,25-(OH)2D3 on proliferation of fibroblast-like synoviocytes and expressions of pro-inflammatory cytokines through regulating MicroRNA-22 in a rat model of rheumatoid arthritis
Citation
Fan, P and He, L and Hu, N and Luo, J and Zhang, J and Mo, LF and Wang, YH and Pu, D and Lv, XH and Hao, ZM and Ding, CH and Xue, WJ and Li, Y, Effect of 1,25-(OH)2D3 on proliferation of fibroblast-like synoviocytes and expressions of pro-inflammatory cytokines through regulating MicroRNA-22 in a rat model of rheumatoid arthritis, Cellular Physiology and Biochemistry, 42, (1) pp. 145-155. ISSN 1015-8987 (2017) [Refereed Article]
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Copyright Statement
Copyright 2017 The Author(s). Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) http://creativecommons.org/licenses/by-nc-nd/4.0/
Abstract
Methods: A rat model of RA was established with a subcutaneous injection of type II collagen. After treated with different concentrations of 1,25-(OH)2D3 the proliferation of FLS was estimated by the MTT method, and the optimal concentration of 1,25-(OH)2D3 was selected for further experiments. Cell proliferation was detected by MTT. Cell cycle and apoptosis were analyzed by FCM. The IL-1β, IL-6, IL-8, and PGE2 protein expressions were determined by ELISA, and MMP-3, INOS, and Cox-2 mRNA expressions were measured by qRT-PCR.
Results: The rat model of RA was successfully established. Compared with the blank group, the 1,25-(OH)2D3 and miR-22 inhibitors groups exhibited higher proliferation inhibition and apoptosis rates, lower levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and PGE2), and decreased mRNA expressions of MMP-3, INOS, and Cox-2. The miR-22 mimics group had lower proliferation inhibition and apoptosis rates, elevated expressions of pro-inflammatory cytokines and MMP-3, INOS, and Cox-2 than the blank group. In contrast to the 1,25-(OH)2D3 group, the proliferation inhibition and apoptosis rates were down-regulated, and the expressions of pro-inflammatory cytokines and MMP-3, INOS, and Cox-2 were up-regulated in the 1,25-(OH)2D3 + miR-22 mimics group.
Conclusion: Our study demonstrated that 1,25-(OH)2D3 inhibits the proliferation of FLS and alleviates inflammatory response in RA rats by down-regulating miR-22.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | 1,25-dihydroxyvitamin D3, collagen induced arthritis, fibroblast-like synoviocytes, MicroRNA-22, pro-inflammatory cytokine, rheumatoid arthritis |
| Research Division: | Medical and Health Sciences |
| Research Group: | Clinical Sciences |
| Research Field: | Rheumatology and Arthritis |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Skeletal System and Disorders (incl. Arthritis) |
| Author: | Ding, CH (Professor Chang-Hai Ding) |
| ID Code: | 124661 |
| Year Published: | 2017 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2018-03-02 |
| Last Modified: | 2018-04-12 |
| Downloads: | 2 View Download Statistics |
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