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Molecular epidemiology of tuberculosis in Tasmania and genomic characterisation of its first known multi-drug resistant case


Gautam, SS and Mac Aogain, M and Cooley, LA and Haug, G and Fyfe, JA and Globan, M and O'Toole, RF, Molecular epidemiology of tuberculosis in Tasmania and genomic characterisation of its first known multi-drug resistant case, PloS one, 13, (2) Article e0192351. ISSN 1932-6203 (2018) [Refereed Article]


Copyright Statement

Copyright 2018 Gautam et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1371/journal.pone.0192351


Background: The origin and spread of tuberculosis (TB) in Tasmania and the types of strains of Mycobacterium tuberculosis complex (MTBC) present in the population are largely unknown.

Objective: The aim of this study was to perform the first genomic analysis of MTBC isolates from Tasmania to better understand the epidemiology of TB in the state.

Methods: Whole-genome sequencing was performed on cultured isolates of MTBC collected from 2014–2016. Single-locus variant analysis was applied to determine the phylogeny of the isolates and the presence of drug-resistance mutations. The genomic data were then cross-referenced against public health surveillance records on each of the cases.

Results: We determined that 83.3% of TB cases in Tasmania from 2014–2016 occurred in non-Australian born individuals. Two possible TB clusters were identified based on single locus variant analysis, one from November-December 2014 (n = 2), with the second from May-August 2015 (n = 4). We report here the first known isolate of multi-drug resistant (MDR) M. tuberculosis in Tasmania from 2016 for which we established its drug resistance mutations and potential overseas origin. In addition, we characterised a case of M. bovis TB in a Tasmanian-born person who presented in 2014, approximately 40 years after the last confirmed case in the state’s bovids.

Conclusions: TB in Tasmania is predominantly of overseas origin with genotypically-unique drug-susceptible isolates of M. tuberculosis. However, the state also exhibits features of TB that are observed in other jurisdictions, namely, the clustering of cases, and drug resistance. Early detection of TB and contact tracing, particularly of overseas-born cases, coordinated with rapid laboratory drug-susceptibility testing and molecular typing, will be essential for Tasmania to reach the World Health Organisation’s TB eradication goals for low-incidence settings.

Item Details

Item Type:Refereed Article
Keywords:mycobacterium tuberculosis, molecular epidemiology, Tasmania, genomic characterisation, multi-drug resistance
Research Division:Biomedical and Clinical Sciences
Research Group:Medical microbiology
Research Field:Medical bacteriology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Gautam, SS (Mr Sanjay Gautam)
UTAS Author:O'Toole, RF (Dr Ronan O'Toole)
ID Code:124454
Year Published:2018
Web of Science® Times Cited:7
Deposited By:Medicine
Deposited On:2018-02-22
Last Modified:2018-12-10
Downloads:96 View Download Statistics

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