University of Tasmania
Browse
ol.2018.7827_AOP_PDF (6).pdf (591.17 kB)

Assessment of CXC ligand 12-mediated calcium signalling and its regulators in basal-like breast cancer cells

Download (591.17 kB)
journal contribution
posted on 2023-05-19, 16:15 authored by Jamaluddin, SYN, Iman AzimiIman Azimi, Davis, FM, Peters, AA, Gonda, TJ, Thompson, EW, Roberts-Thomson, SJ, Monteith, GR
CXC ligand (L)12 is a chemokine implicated in the migration, invasion and metastasis of cancer cells via interaction with its receptors CXC chemokine receptor (CXCR)4 and CXCR7. In the present study, CXCL12‑mediated Ca2+ signalling was compared with two basal‑like breast cancer cell lines, MDA‑MB‑231 and MDA‑MB‑468, which demonstrate distinct metastatic potential. CXCL12 treatment induced Ca2+ responses in the more metastatic MDA‑MB‑231 cells but not in the less metastatic MDA‑MB‑468 cells. Assessment of mRNA levels of CXCL12 receptors and their potential modulators in both cell lines revealed that CXCR4 and CXCR7 levels were increased in MDA‑MB‑231 cells compared with MDA‑MB‑468 cells. Cluster of differentiation (CD)24, the negative regulator of CXCL12 responses, demonstrated increased expression in MDA‑MB‑468 cells compared with MDA‑MB‑231 cells, and the two cell lines expressed comparable levels of hypoxia‑inducible factor (HIF)2α, a CXCR4 regulator. Induction of epithelial-mesenchymal transition (EMT) by epidermal growth factor exhibited opposite effects on CXCR4 mRNA levels compared with hypoxia‑induced EMT. Neither EMT inducer exhibited an effect on CXCR7 expression, however hypoxia increased HIF2α expression levels in MDA‑MB‑468 cells. Analysis of the gene expression profiles of breast tumours revealed that the highest expression levels of CXCR4 and CXCR7 were in the Claudin‑Low molecular subtype, which is markedly associated with EMT features.

History

Publication title

Oncology Letters

Volume

15

Issue

4

Pagination

4289-4295

ISSN

1792-1074

Department/School

School of Pharmacy and Pharmacology

Publisher

Spandidos Publications

Place of publication

Greece

Rights statement

Copyright Jamaludin et al. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/

Repository Status

  • Open

Socio-economic Objectives

Clinical health not elsewhere classified

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC