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Nitric oxide is required for the insulin sensitizing effects of contraction in mouse skeletal muscle


Zhang, X and Hiam, D and Hong, YH and Zulli, A and Hayes, A and Rattigan, S and McConell, GK, Nitric oxide is required for the insulin sensitizing effects of contraction in mouse skeletal muscle, Journal of Physiology, 595, (24) pp. 7427-7439. ISSN 0022-3751 (2017) [Refereed Article]

Copyright Statement

© 2017 The Authors. The Journal of Physiology © 2017 The Physiological Society

DOI: doi:10.1113/JP275133


The factors regulating the increase in skeletal muscle insulin sensitivity after exercise are unclear. We examined whether nitric oxide (NO) is required for the increase in insulin sensitivity after ex vivo contractions. Isolated C57BL/6J mouse EDL muscles were contracted for 10 min or remained at rest (basal) with or without the NO synthase (NOS) inhibition (NG -monomethyl-L-arginine; L-NMMA; 100 μm). Then, 3.5 h post contraction/basal, muscles were exposed to saline or insulin (120 μU ml-1 ) with or without L-NMMA during the last 30 min. L-NMMA had no effect on basal skeletal muscle glucose uptake. The increase in muscle glucose uptake with insulin (57%) was significantly (P < 0.05) greater after prior contraction (140% increase). NOS inhibition during the contractions had no effect on this insulin-sensitizing effect of contraction, whereas NOS inhibition during insulin prevented the increase in skeletal muscle insulin sensitivity post-contraction. Soluble guanylate cyclase inhibition, protein kinase G (PKG) inhibition or cyclic nucleotide phosphodiesterase inhibition each had no effect on the insulin-sensitizing effect of prior contraction. In conclusion, NO is required for increases in insulin sensitivity several hours after contraction of mouse skeletal muscle via a cGMP/PKG independent pathway.

Item Details

Item Type:Refereed Article
Keywords:L-NMMA, insulin sensitivity, muscle contraction
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Endocrinology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Rattigan, S (Professor Stephen Rattigan)
ID Code:124236
Year Published:2017
Web of Science® Times Cited:4
Deposited By:Menzies Institute for Medical Research
Deposited On:2018-02-14
Last Modified:2018-07-23

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