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Vibrioferrin production by the food spoilage bacterium Pseudomonas fragi

Citation

Stanborough, T and Fegan, N and Powell, SM and Tamplin, M and Chandry, PS, Vibrioferrin production by the food spoilage bacterium Pseudomonas fragi, FEMS Microbiology Letters, 365, (6) pp. 1-27. ISSN 0378-1097 (2018) [Refereed Article]


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Copyright Statement

Copyright FEMS 2017. This is a pre-copyedited, author-produced version of an article accepted for publication in FEMS microbiology letters following peer review. The version of record, Stanborough, T., Fegan, N., Powell, S. M., Tamplin, M., Chandry, P. S., 2018. Vibrioferrin production by the food spoilage bacterium Pseudomonas fragi, FEMS microbiology letters, 365(6), 1-27, is available online at: http://dx.doi.org/10.1093/femsle/fnx279

DOI: doi:10.1093/femsle/fnx279

Abstract

Pseudomonas fragi is a meat and milk spoilage bacterium with high iron requirements, however mechanisms of iron acquisition remain largely unknown. The aim of this work was to investigate siderophore production as an iron acquisition system for P. fragi. A vibrioferrin siderophore gene cluster was identified in 13 P. fragi and experiments were conducted with a representative strain of this group (F1801). Chromeazurol S assays showed P. fragi F1801 produced siderophores under iron starvation at optimum growth and refrigeration temperature. Conversely, supplementation of low iron media with 50 M FeCl3 repressed transcription of the vibrioferrin genes and siderophore production. Disruption of the siderophore receptor (pvuA) caused polar effects on downstream vibrioferrin genes, resulting in impaired siderophore production of the ΔpvuA mutant. Growth of this mutant was compared to growth of a control strain (Δlip) with wild-type vibrioferrin genes in low iron media supplemented with iron chelators 2,2-bipyridyl or apo-transferrin. While 25 M 2,2- bipyridyl caused impaired growth of ΔpvuA, growth of the mutant was completely inhibited by 2.5 M apo-transferrin, but could be restored by FeCl3- addition. In summary, this work identifies a vibrioferrin-mediated iron acquisition system of P. fragi, which is required for growth of this bacterium under iron-starvation.

Item Details

Item Type:Refereed Article
Keywords:Pseudomonas, spoilage, siderophore
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Stanborough, T (Ms Tamsyn Stanborough)
Author:Powell, SM (Dr Shane Powell)
Author:Tamplin, M (Professor Mark Tamplin)
ID Code:123894
Year Published:2018
Deposited By:Agriculture and Food Systems
Deposited On:2018-01-31
Last Modified:2018-08-03
Downloads:0

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