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Developmental and gut-related changes to microbiomes of the cultured juvenile spiny lobster Panulirus ornatus

Citation

Ooi, MC and Goulden, EF and Smith, GG and Nowak, BF and Bridle, AR, Developmental and gut-related changes to microbiomes of the cultured juvenile spiny lobster Panulirus ornatus, FEMS Microbiology Ecology, 93, (12) Article fix159. ISSN 0168-6496 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 FEMS

DOI: doi:10.1093/femsec/fix159

Abstract

With recent technologies making it possible for commercial scale closed life-cycle aquaculture production of spiny lobster (Panulirus ornatus) comes a strong impetus to further understand aspects of lobster health. The gut microbiome plays a crucial role in host health, affecting growth, digestion, immune responses and pathogen resistance. Herein we characterise and compare gut microbiomes across different developmental stages (67 days post-emergence [dpe], 52 dpe and 13 months post-emergence [mpe]) and gut regions (foregut, midgut and hindgut) of cultured P. ornatus juveniles. Gut samples were analysed using 16S rRNA next-generation sequencing. Core gut microbiomes of P. ornatus comprised the phyla Tenericutes and Proteobacteria. Within class Gammaproteobacteria, families Pseudoalteromonadaceae and Vibrionaceae were dominant members across the majority of the gut microbiomes. Characterisation of bacterial communities from 13 mpe lobsters indicated that the hindgut microbiome was more diverse and compositionally dissimilar to the foregut and midgut. The bacterial composition of the hindgut was more similar among younger juveniles (67 dpe and 52 dpe) compared to 13 mpe lobsters. This is the first study to explore gut microbiomes of spiny lobster juveniles. We demonstrate that the composition of the gut microbiome was shaped by gut region, whereas the structure of the hindgut microbiome was influenced by developmental stage.

Item Details

Item Type:Refereed Article
Keywords:cultured spiny lobster, gut microbiome, Panulirus ornatus, microbiology, developmental stage, gut region, Tenericutes, Proteobacteria
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Ooi, MC (Miss Mei Ooi)
Author:Goulden, EF (Dr Evan Goulden)
Author:Smith, GG (Associate Professor Gregory Smith)
Author:Nowak, BF (Professor Barbara Nowak)
Author:Bridle, AR (Dr Andrew Bridle)
ID Code:123656
Year Published:2017
Funding Support:Australian Research Council (IH120100032)
Deposited By:Fisheries and Aquaculture
Deposited On:2018-01-17
Last Modified:2018-03-22
Downloads:0

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