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Using SSR markers for hybrid identification and resource management in Vietnamese Acacia breeding programs


Le, S and Harwood, CE and Griffin, AR and Do, SH and Ha, TH and Ratnam, W and Vaillancourt, RE, Using SSR markers for hybrid identification and resource management in Vietnamese Acacia breeding programs, Tree Genetics and Genomes, 13, (5) Article 102. ISSN 1614-2942 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 Springer-Verlag GmbH Germany

DOI: doi:10.1007/s11295-017-1184-2


We used a set of 16 SSR markers to check the identity of pure-species and hybrid clones in Vietnamís Acacia auriculiformis, Acacia mangium, and acacia hybrid (A. mangiumA. auriculiformis) breeding programs. The statistics package HIest, applied to a large synthesized population, enabled accurate allocation of genotypes to the two pure species, F1 and F2 inter-specific hybrids and backcrosses, based on estimates of hybridity and heterozygosity. The hybridity status of putatively pure A. mangium and A. auriculiformis clones in adjacent clonal seed orchards was checked. Four out of 100 clones selected as A. mangium were found to be backcrosses (A. mangium ◊ F1 inter-specific hybrid) while out of 96 clones selected as A. auriculiformis, two were F1 hybrids and two were backcrosses (A. auriculiformis ◊ F1 hybrid). The markers were then applied to check the hybridity status of 160 putative acacia F1 hybrid genotypes that had been selected on morphological criteria from open-pollinated progenies collected from A. auriculiformis and A. mangium parents. Many selections based on morphology were found to be mistaken. Only thirteen of 63 clones originating from A. auriculiformis mothers were F1 hybrids, four were backcrosses, and the remaining 46 were pure A. auriculiformis. Fewer mistakes were evident for clones selected from A. mangium mothers, with 82 out of 89 clones confirmed as F1 hybrids, three as backcrosses, and four as pure A. mangium. The occurrence of F1 hybrids and backcrosses in pure-species seed orchards and their progeny shows that inter-species contamination is an issue requiring management in both pure-species and in hybrid breeding of these species in Vietnam. Examination of genetic distances among verified clones showed patterns of relatedness that were consistent with pedigree records. Implications for resource management as well as for breeding and clonal selection strategies are considered.

Item Details

Item Type:Refereed Article
Keywords:Acacia, microsatellite, Acacia auriculiformis, Acacia mangium, acacia hybrid, SSR markers, hybrid detection, hybridity
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Forestry sciences
Research Field:Tree improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Forestry
Objective Field:Hardwood plantations
UTAS Author:Le, S (Mr Son Le)
UTAS Author:Harwood, CE (Mr Christopher Harwood)
UTAS Author:Griffin, AR (Professor Rod Griffin)
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
ID Code:123467
Year Published:2017
Web of Science® Times Cited:7
Deposited By:Plant Science
Deposited On:2018-01-09
Last Modified:2018-05-10

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