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Assessment of the binding performance of histamine-imprinted microspheres by frontal analysis capillary electrophoresis


Romano, EF and Quirino, JP and Holdsworth, JL and So, RC and Holdsworth, CI, Assessment of the binding performance of histamine-imprinted microspheres by frontal analysis capillary electrophoresis, Electrophoresis, 38, (9-10) pp. 1251-1259. ISSN 0173-0835 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/elps.201600448


Frontal analysis capillary electrophoresis was used to evaluate the binding performance of molecularly imprinted microspheres (MIM) toward its template histamine and analogs at pH 7, and compared to the high performance liquid chromatographic method. In both methods, batch binding was employed and the binding parameters were calculated from the measured concentration of unbound amine analytes and afforded comparable histamine equilibrium dissociation constants (Kd ∼ 0.4 mM). FACE was easily carried out at shorter binding equilibration time (i.e. 30 min) and without the need to separate the microspheres, circumventing laborious and, in the case of the system under study, inefficient sample filtration. It also allowed for competitive binding studies by virtue of its ability to distinctly separate intact microspheres and all tested amines which could not be resolved in HPLC. Kdís for nonimprinted (control) microspheres (NIM) from FACE and HPLC were also comparable (∼ 0.6 mM) but at higher histamine concentrations, HPLC gave lower histamine binding. This discrepancy was attributed to inefficient filtration of the batch binding samples prior to HPLC analysis resulting in an over-estimation of the concentration of free histamine brought about by the presence of unfiltered histamine-bound microspheres.

Item Details

Item Type:Refereed Article
Keywords:materials, capillary electrophoresis, frontal analysis capillary electrophoresis, histamine, histamine-imprinted microspheres, molecularly imprinted polymers
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:123456
Year Published:2017
Web of Science® Times Cited:4
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2018-01-09
Last Modified:2019-08-06

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