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Cell-based phenotyping reveals QTL for membrane potential maintenance associated with hypoxia and salinity stress tolerance in barley
Citation
Gill, MB and Zeng, F and Shabala, L and Zhang, G and Fan, Y and Shabala, S and Zhou, M, Cell-based phenotyping reveals QTL for membrane potential maintenance associated with hypoxia and salinity stress tolerance in barley, Frontiers in Plant Science, 8 Article 1941. ISSN 1664-462X (2017) [Refereed Article]
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Copyright Statement
2017 Gill, Zeng, Shabala, Zhang, Fan, Shabala and Zhou. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.3389/fpls.2017.01941
Abstract
Waterlogging and salinity are two major abiotic stresses that hamper crop production world-wide resulting in multibillion losses. Plant abiotic stress tolerance is conferred by many interrelated mechanisms. Amongst these, the cell’s ability to maintain membrane potential (MP) is considered to be amongst the most crucial traits, a positive relationship between the ability of plants to maintain highly negative MP and its tolerance to both salinity and waterlogging stress. However, no attempts have been made to identify quantitative trait loci (QTL) conferring this trait. In this study, the microelectrode MIFE technique was used to measure the plasma membrane potential of epidermal root cells of 150 double haploid (DH) lines of barley (Hordeum vulgare L.) from a cross between a Chinese landrace TX9425 and Japanese malting cultivar Naso Nijo under hypoxic conditions. A major QTL for the MP in the epidermal root cells in hypoxia-exposed plants was identified. This QTL was located on 2H, at a similar position to the QTL for waterlogging and salinity tolerance reported in previous studies. Further analysis confirmed that MP showed a significant contribution to both waterlogging and salinity tolerance. The fact that the QTL for MP was controlled by a single major QTL illustrates the power of the single-cell phenotyping approach and opens prospects for fine mapping this QTL and thus being more effective in marker assisted selection.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | hypoxia, salinity, membrane potential, Hordeum vulgare, waterlogging tolerance |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant physiology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Grains and seeds |
| Objective Field: | Barley |
| UTAS Author: | Gill, MB (Mr Muhammad Gill) |
| UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
| UTAS Author: | Fan, Y (Miss Yun Fan) |
| UTAS Author: | Shabala, S (Professor Sergey Shabala) |
| UTAS Author: | Zhou, M (Professor Meixue Zhou) |
| ID Code: | 122883 |
| Year Published: | 2017 |
| Web of Science® Times Cited: | 21 |
| Deposited By: | Agriculture and Food Systems |
| Deposited On: | 2017-12-05 |
| Last Modified: | 2018-06-06 |
| Downloads: | 87 View Download Statistics |
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