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Cell-based phenotyping reveals QTL for membrane potential maintenance associated with hypoxia and salinity stress tolerance in barley


Gill, MB and Zeng, F and Shabala, L and Zhang, G and Fan, Y and Shabala, S and Zhou, M, Cell-based phenotyping reveals QTL for membrane potential maintenance associated with hypoxia and salinity stress tolerance in barley, Frontiers in Plant Science, 8 Article 1941. ISSN 1664-462X (2017) [Refereed Article]


Copyright Statement

2017 Gill, Zeng, Shabala, Zhang, Fan, Shabala and Zhou. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.3389/fpls.2017.01941


Waterlogging and salinity are two major abiotic stresses that hamper crop production world-wide resulting in multibillion losses. Plant abiotic stress tolerance is conferred by many interrelated mechanisms. Amongst these, the cellís ability to maintain membrane potential (MP) is considered to be amongst the most crucial traits, a positive relationship between the ability of plants to maintain highly negative MP and its tolerance to both salinity and waterlogging stress. However, no attempts have been made to identify quantitative trait loci (QTL) conferring this trait. In this study, the microelectrode MIFE technique was used to measure the plasma membrane potential of epidermal root cells of 150 double haploid (DH) lines of barley (Hordeum vulgare L.) from a cross between a Chinese landrace TX9425 and Japanese malting cultivar Naso Nijo under hypoxic conditions. A major QTL for the MP in the epidermal root cells in hypoxia-exposed plants was identified. This QTL was located on 2H, at a similar position to the QTL for waterlogging and salinity tolerance reported in previous studies. Further analysis confirmed that MP showed a significant contribution to both waterlogging and salinity tolerance. The fact that the QTL for MP was controlled by a single major QTL illustrates the power of the single-cell phenotyping approach and opens prospects for fine mapping this QTL and thus being more effective in marker assisted selection.

Item Details

Item Type:Refereed Article
Keywords:hypoxia, salinity, membrane potential, Hordeum vulgare, waterlogging tolerance
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Gill, MB (Mr Muhammad Gill)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Fan, Y (Miss Yun Fan)
UTAS Author:Shabala, S (Professor Sergey Shabala)
UTAS Author:Zhou, M (Professor Meixue Zhou)
ID Code:122883
Year Published:2017
Web of Science® Times Cited:21
Deposited By:Agriculture and Food Systems
Deposited On:2017-12-05
Last Modified:2018-06-06
Downloads:87 View Download Statistics

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