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Expressing Arabidopsis thaliana V-ATPase subunit C in barley (Hordeum vulgare) improves plant performance under saline condition by enabling better osmotic adjustment
Citation
Adem, GD and Roy, SJ and Huang, Y and Chen, Z-H and Wang, F and Zhou, M and Bowman, JP and Holford, P and Shabala, SN, Expressing Arabidopsis thaliana V-ATPase subunit C in barley (Hordeum vulgare) improves plant performance under saline condition by enabling better osmotic adjustment, Functional Plant Biology, 44, (12) pp. 1147-1159. ISSN 1445-4408 (2017) [Refereed Article]
Copyright Statement
Copyright 2017 CSIRO
DOI: doi:10.1071/FP17133
Abstract
Salinity is a global problem affecting agriculture that results in an estimated US$27 billion loss in revenue per year. Overexpression of vacuolar ATPase subunits has been shown to be beneficial in improving plant performance under saline conditions. Most studies, however, have not shown whether overexpression of genes encoding ATPase subunits results in improvements in grain yield, and have not investigated the physiological mechanisms behind the improvement in plant growth. In this study, we constitutively expressed Arabidopsis Vacuolar ATPase subunit C (AtVHA-C) in barley. Transgenic plants were assessed for agronomical and physiological characteristics, such as fresh and dry biomass, leaf pigment content, stomatal conductance, grain yield, and leaf Na+ and K+ concentration, when grown in either 0 or 300 mM NaCl. When compared with non-transformed barley, AtVHA-C expressing barley lines had a smaller reduction in both biomass and grain yield under salinity stress. The transgenic lines accumulated Na+ and K+ in leaves for osmotic adjustment. This in turn saves energy consumed in the synthesis of organic osmolytes that otherwise would be needed for osmotic adjustment.
Item Details
Item Type: | Refereed Article |
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Keywords: | salinity, osmotic adjustment, stress, organic osmolytes, potassium, salinity stress tolerance, sodium, vacuolar sequestration |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Grains and seeds |
Objective Field: | Barley |
UTAS Author: | Adem, GD (Mr Getnet Adem) |
UTAS Author: | Wang, F (Miss Feifei Wang) |
UTAS Author: | Zhou, M (Professor Meixue Zhou) |
UTAS Author: | Bowman, JP (Associate Professor John Bowman) |
UTAS Author: | Shabala, SN (Professor Sergey Shabala) |
ID Code: | 122882 |
Year Published: | 2017 |
Web of Science® Times Cited: | 15 |
Deposited By: | Agriculture and Food Systems |
Deposited On: | 2017-12-05 |
Last Modified: | 2018-06-06 |
Downloads: | 0 |
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