File(s) under permanent embargo
Expressing Arabidopsis thaliana V-ATPase subunit C in barley (Hordeum vulgare) improves plant performance under saline condition by enabling better osmotic adjustment
journal contribution
posted on 2023-05-19, 14:00 authored by Adem, GD, Roy, SJ, Huang, Y, Chen, Z-H, Wang, F, Meixue ZhouMeixue Zhou, John BowmanJohn Bowman, Holford, P, Sergey ShabalaSergey ShabalaSalinity is a global problem affecting agriculture that results in an estimated US$27 billion loss in revenue per year. Overexpression of vacuolar ATPase subunits has been shown to be beneficial in improving plant performance under saline conditions. Most studies, however, have not shown whether overexpression of genes encoding ATPase subunits results in improvements in grain yield, and have not investigated the physiological mechanisms behind the improvement in plant growth. In this study, we constitutively expressed Arabidopsis Vacuolar ATPase subunit C (AtVHA-C) in barley. Transgenic plants were assessed for agronomical and physiological characteristics, such as fresh and dry biomass, leaf pigment content, stomatal conductance, grain yield, and leaf Na+ and K+ concentration, when grown in either 0 or 300 mM NaCl. When compared with non-transformed barley, AtVHA-C expressing barley lines had a smaller reduction in both biomass and grain yield under salinity stress. The transgenic lines accumulated Na+ and K+ in leaves for osmotic adjustment. This in turn saves energy consumed in the synthesis of organic osmolytes that otherwise would be needed for osmotic adjustment.
History
Publication title
Functional Plant BiologyVolume
44Issue
12Pagination
1147-1159ISSN
1445-4408Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
C S I R O PublishingPlace of publication
150 Oxford St, Po Box 1139, Collingwood, Australia, Victoria, 3066Rights statement
Copyright 2017 CSIRORepository Status
- Restricted