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Chemotyping of new hop (Humulus lupulus L.) genotypes using comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry

Citation

Yan, D and Wong, YF and Tedone, L and Shellie, RA and Marriott, PJ and Whittock, SP and Koutoulis, A, Chemotyping of new hop (Humulus lupulus L.) genotypes using comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry, Journal of Chromatography A, 1536 pp. 110-121. ISSN 0021-9673 (2018) [Refereed Article]

Copyright Statement

Copyright 2017 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2017.08.020

Abstract

Comprehensive two-dimensional gas chromatography with quadrupole accurate mass time-of-flight mass spectrometry (GC GC-Q-TOFMS) is employed to profile Humulus lupulus L. (hop) essential oils. Comparison of characterised essential oils allows discrimination among chemotypes. Experimental and commercial hop genotypes displayed distinguishable chemotypic patterns among the volatile secondary metabolites making up their essential oils. In total, 210306 unique compounds were detected (depending on specific genotype), with 99 of these compounds either positively or tentatively identified. Identified volatile secondary metabolites were grouped into esters, monoterpene hydrocarbons, oxygenated onoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes and ketones. Terpenoids were the dominant chemical families across all hop genotypes analysed, representing between 67% and 90% of the total ion count. The multidimensional chromatographic profiles of hop essential oils are extremely information-rich, making GC GC-Q-TOFMS useful for fast screening of new hybrid hop genotypes, and therefore informing breeding strategies to derive new commercial hop cultivars for the development of distinctive and desirable beers.

Item Details

Item Type:Refereed Article
Keywords:hop, Humulus lupulus L., comprehensive two-dimensional gas chromatography, accurate mass time-of-flight mass spectrometry, hop chemotypes, hop genotypes
Research Division:Agricultural and Veterinary Sciences
Research Group:Horticultural Production
Research Field:Post Harvest Horticultural Technologies (incl. Transportation and Storage)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Hops
Author:Yan, D (Ms Dandan Yan)
Author:Tedone, L (Ms Laura Tedone)
Author:Shellie, RA (Associate Professor Robert Shellie)
Author:Whittock, SP (Mr Simon Whittock)
Author:Koutoulis, A (Professor Anthony Koutoulis)
ID Code:122874
Year Published:2018
Web of Science® Times Cited:3
Deposited By:Plant Science
Deposited On:2017-12-04
Last Modified:2018-03-19
Downloads:0

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