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Isolation of alginate lyase-producing bacteria and screening for their potential characteristics as abalone probionts
Citation
Amin, M and Bolch, CJ and Adams, MB and Burke, CM, Isolation of alginate lyase-producing bacteria and screening for their potential characteristics as abalone probionts, Aquaculture Research, 48, (11) pp. 5614-5623. ISSN 1355-557X (2017) [Refereed Article]
Copyright Statement
Copyright 2017 John Wiley & Sons Ltd
Abstract
This study is aimed at the isolation and screening of alginate lyase-producing bacteria from the gastrointestinal tracts of hybrid abalone, Haliotis rubra x H. laevigata, as probiotic candidates. Six bacterial isolates were detected to produce alginate lyase. Of these, the isolate with the highest alginate-lyase activity was identified as Enterobacter ludwigii strain EN-119, displaying 99% similarity of 16S rDNA sequence. Further assays indicated that E. ludwigii showed good viability and stability when it was incorporated into manufactured pellets and stored at 4°C for 7 days. The isolate also had high tolerance of high salinity (35 mg/L), low pH in simulated stomach juice (5) and to simulated intestinal juice containing surfactants such as bile salts and gastric enzymes (pepsin and trypsin). Additionally, a short, preliminary study indicated that supplementation of E. ludwigii via manufactured pellets improved the total weight gain and specific growth rate of hybrid abalone. These results suggest that E. ludwigii is a potential probiont for shortening the culture period of hybrid abalone.
Item Details
Item Type: | Refereed Article |
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Keywords: | hybrid abalone, probiotic candidate, alginate lyase, Enterobacter ludwigii |
Research Division: | Agricultural, Veterinary and Food Sciences |
Research Group: | Fisheries sciences |
Research Field: | Aquaculture |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Fisheries - aquaculture |
Objective Field: | Fisheries - aquaculture not elsewhere classified |
UTAS Author: | Amin, M (Mr Muhamad Amin) |
UTAS Author: | Bolch, CJ (Associate Professor Christopher Bolch) |
UTAS Author: | Adams, MB (Dr Mark Adams) |
UTAS Author: | Burke, CM (Dr Chris Burke) |
ID Code: | 122551 |
Year Published: | 2017 |
Web of Science® Times Cited: | 7 |
Deposited By: | Fisheries and Aquaculture |
Deposited On: | 2017-11-17 |
Last Modified: | 2018-03-22 |
Downloads: | 0 |
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