This study is aimed at the isolation and screening of alginate lyase-producing bacteria from the gastrointestinal tracts of hybrid abalone, Haliotis rubra x H. laevigata, as probiotic candidates. Six bacterial isolates were detected to produce alginate lyase. Of these, the isolate with the highest alginate-lyase activity was identified as Enterobacter ludwigii strain EN-119, displaying 99% similarity of 16S rDNA sequence. Further assays indicated that E. ludwigii showed good viability and stability when it was incorporated into manufactured pellets and stored at 4°C for 7 days. The isolate also had high tolerance of high salinity (35 mg/L), low pH in simulated stomach juice (5) and to simulated intestinal juice containing surfactants such as bile salts and gastric enzymes (pepsin and trypsin). Additionally, a short, preliminary study indicated that supplementation of E. ludwigii via manufactured pellets improved the total weight gain and specific growth rate of hybrid abalone. These results suggest that E. ludwigii is a potential probiont for shortening the culture period of hybrid abalone.

Item Type:	Refereed Article
Keywords:	hybrid abalone, probiotic candidate, alginate lyase, Enterobacter ludwigii
Research Division:	Agricultural, Veterinary and Food Sciences
Research Group:	Fisheries sciences
Research Field:	Aquaculture
Objective Division:	Animal Production and Animal Primary Products
Objective Group:	Fisheries - aquaculture
Objective Field:	Fisheries - aquaculture not elsewhere classified
UTAS Author:	Amin, M (Mr Muhamad Amin)
UTAS Author:	Bolch, CJ (Associate Professor Christopher Bolch)
UTAS Author:	Adams, MB (Dr Mark Adams)
UTAS Author:	Burke, CM (Dr Chris Burke)
ID Code:	122551
Year Published:	2017
Web of Science® Times Cited:	7
Deposited By:	Fisheries and Aquaculture
Deposited On:	2017-11-17
Last Modified:	2018-03-22
Downloads:	0