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Isolation of alginate lyase-producing bacteria and screening for their potential characteristics as abalone probionts


Amin, M and Bolch, CJ and Adams, MB and Burke, CM, Isolation of alginate lyase-producing bacteria and screening for their potential characteristics as abalone probionts, Aquaculture Research, 48, (11) pp. 5614-5623. ISSN 1355-557X (2017) [Refereed Article]

Copyright Statement

Copyright 2017 John Wiley & Sons Ltd

DOI: doi:10.1111/are.13383


This study is aimed at the isolation and screening of alginate lyase-producing bacteria from the gastrointestinal tracts of hybrid abalone, Haliotis rubra x H. laevigata, as probiotic candidates. Six bacterial isolates were detected to produce alginate lyase. Of these, the isolate with the highest alginate-lyase activity was identified as Enterobacter ludwigii strain EN-119, displaying 99% similarity of 16S rDNA sequence. Further assays indicated that E. ludwigii showed good viability and stability when it was incorporated into manufactured pellets and stored at 4°C for 7 days. The isolate also had high tolerance of high salinity (35 mg/L), low pH in simulated stomach juice (5) and to simulated intestinal juice containing surfactants such as bile salts and gastric enzymes (pepsin and trypsin). Additionally, a short, preliminary study indicated that supplementation of E. ludwigii via manufactured pellets improved the total weight gain and specific growth rate of hybrid abalone. These results suggest that E. ludwigii is a potential probiont for shortening the culture period of hybrid abalone.

Item Details

Item Type:Refereed Article
Keywords:hybrid abalone, probiotic candidate, alginate lyase, Enterobacter ludwigii
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Fisheries - aquaculture not elsewhere classified
UTAS Author:Amin, M (Mr Muhamad Amin)
UTAS Author:Bolch, CJ (Associate Professor Christopher Bolch)
UTAS Author:Adams, MB (Dr Mark Adams)
UTAS Author:Burke, CM (Dr Chris Burke)
ID Code:122551
Year Published:2017
Web of Science® Times Cited:7
Deposited By:Fisheries and Aquaculture
Deposited On:2017-11-17
Last Modified:2018-03-22

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