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Further Investigations of the Effects of Anti-β2GP1 Antibodies on Collagen-Induced Platelet Aggregation

Citation

Ho, YC and Ahuja, KDK and Adams, MJ, Further Investigations of the Effects of Anti-β2GP1 Antibodies on Collagen-Induced Platelet Aggregation, Clinical and Applied Thrombosis - Hemostasis Article Epub ahead of print. ISSN 1076-0296 (2017) [Refereed Article]


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DOI: doi:10.1177/1076029617736384

Abstract

Anti-beta-2-glycoprotein 1 (anti-β2GP1) antibodies are associated with increased thrombotic risk in patients with autoimmune disease. There is conflicting evidence on the effects of anti-β2GP1 antibodies on platelets, with both enhanced and inhibited aggregation previously reported. However, previous studies did not include isotype antibodies to ensure the observed effects were due to anti-β2GP1 antibodies. The aims of this study were to (1) investigate the effects of anti-β2GP1 antibodies on collagen-induced platelet aggregation in parallel with negative control (buffer normal saline) and isotype control antibodies and (2) determine the lupus anticoagulant (LA) activity of anti-β2GP1 antibodies used. Three animal-derived anti-human-β2GP1 antibodies (1.25, 2.5, and 5 μg/mL) incubated with healthy platelet-rich plasma were activated by collagen (2.5 μg/mL). Each anti-β2GP1 antibody demonstrated the inhibition of aggregation compared to negative control, but not to isotype control. No anti-β2GP1 antibody demonstrated LA activity, suggesting they were probably nonpathological. This study highlights both negative and isotype control markers are important to validate the effects of anti-β2GP1 antibodies. Assays to measure anti-domain I-β2GP1 antibodies are recommended to be used in conjunction with functional measures to further investigate the effects of anti-β2GP1 antibodies.

Item Details

Item Type:Refereed Article
Keywords:platelets, Antiphospholoipid syndrome
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Haematology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
Author:Ho, YC (Mr Yik Ho)
Author:Ahuja, KDK (Dr Kiran Ahuja)
Author:Adams, MJ (Dr Murray Adams)
ID Code:122474
Year Published:2017
Deposited By:Health Sciences
Deposited On:2017-11-15
Last Modified:2017-11-16
Downloads:0

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