eCite Digital Repository
Live-Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy
Citation
Kisler, K and Dominguez, R, Live-Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy, Estrogen Receptors: Methods and Protocols, Humana Press, KM Eyster (ed), New York, pp. 175-187. ISBN 978-1-4939-3127-9 (2016) [Research Book Chapter]
Copyright Statement
Copyright 2016 Springer Science+Business Media New York
DOI: doi:10.1007/978-1-4939-3127-9_14
Abstract
© Springer Science+Business Media New York 2016. Trafficking studies of plasma membrane-localized intracellular estrogen receptors have mainly relied on biochemical and histological techniques to locate the receptor before and after estradiol stimulation. More often than not these experiments were performed using postmortem, lysed, or fixed tissue samples, whose tissue or cellular structure is typically severely altered or at times completely lost, making the definitive localization of estrogen receptors difficult to ascertain. To overcome this limitation we began using total internal reflection fluorescence microscopy (TIRFM) to study the trafficking of plasma membrane estrogen receptors. This real-time imaging approach, described in this chapter, permits observation of live, intact cells while allowing visualization of the steps (in time and spatial distribution) involved in receptor activation by estradiol and movements on and near the membrane. TIRFM yields high-contrast real-time images of fluorescently labeled E6BSA molecules on and just below the cell surface and is ideal for studying estrogen receptor trafficking in living cells.
Item Details
Item Type: | Research Book Chapter |
---|---|
Keywords: | Live cell imaging, TIRF, trafficking, estrogen receptor |
Research Division: | Biological Sciences |
Research Group: | Zoology |
Research Field: | Animal cell and molecular biology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Dominguez, R (Dr Reymundo Dominguez) |
ID Code: | 122239 |
Year Published: | 2016 |
Deposited By: | Medicine |
Deposited On: | 2017-11-06 |
Last Modified: | 2018-03-20 |
Downloads: | 0 |
Repository Staff Only: item control page