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17-β-Estradiol-mediated activation of extracellular-signal regulated kinase, phosphatidylinositol 3-kinase/protein kinase B-Akt and N-methyl-α-aspartate receptor phosphorylation in cortical synaptoneurosomes

Citation

Dominguez, R and Liu, R and Baudry, M, 17-β-Estradiol-mediated activation of extracellular-signal regulated kinase, phosphatidylinositol 3-kinase/protein kinase B-Akt and N-methyl-α-aspartate receptor phosphorylation in cortical synaptoneurosomes, Journal of Neurochemistry, 10, (1) pp. 232-240. ISSN 0022-3042 (2007) [Refereed Article]


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DOI: doi:10.1111/j.1471-4159.2006.04360.x

Abstract

In addition to its well-known activational mechanism, the steroid hormone 17-β-estradiol (E2) has been shown to rapidly activate various signal transduction pathways that could participate in estrogen-mediated regulation of synaptic plasticity. Although the mechanisms underlying these effects are not clearly understood, it has been repeatedly suggested that they involve a plasma membrane receptor which has direct links to several intracellular signaling cascades. To further address the question of whether E2 acts directly at the synapse and through membrane-bound receptors, we studied the effects of E2 and of ligands of estrogen receptors on various signaling pathways in cortical synaptoneurosomes. Our results demonstrate that E2 elicits N-methyl-Daspartate receptor phosphorylation and activates the extracellular signal-regulated kinase and the phosphatidylinositol 3-kinase/Akt signal transduction pathways in this cortical membrane preparation. Furthermore, we provide evidence for the presence of a membrane-bound estrogen receptor responsible for these effects in cortical synaptoneurosomes. Our study demonstrates that E2 directly acts at cortical synapses, and that synaptoneurosomes provide a useful system to investigate the mechanisms by which E2 regulates synaptic transmission and plasticity.

Item Details

Item Type:Refereed Article
Keywords:Estrogen, signal transduction, ERK, Akt, synaptoneurosomes
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Signal Transduction
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Dominguez, R (Dr Reymundo Dominguez)
ID Code:122234
Year Published:2007
Web of Science® Times Cited:41
Deposited By:Medicine (Discipline)
Deposited On:2017-11-06
Last Modified:2017-11-14
Downloads:0

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