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A New Method for Targeted and Sustained Induction of Type 2 Diabetes in Rodents
Citation
Premilovac, D and Gasperini, RJ and Sawyer, S and West, A and Keske, M and Taylor, BV and Foa, L, A New Method for Targeted and Sustained Induction of Type 2 Diabetes in Rodents, Scientific Reports, 7 Article 14158. ISSN 2045-2322 (2017) [Refereed Article]
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Copyright Statement
Copyright 2017 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1038/s41598-017-14114-4
Abstract
Type 2 diabetes is a chronic metabolic disorder that is becoming a leading cause of morbidity and mortality. The prolonged time-course of human type 2 diabetes makes modelling of the disease difficult and additional animal models and methodologies are needed. The goal of this study was to develop and characterise a new method that allows controlled, targeted and sustained induction of discrete stages of type 2 diabetes in rodents. Using adult, male rats, we employed a three-week high fat-diet regimen and confirmed development of obesity-associated glucose intolerance, a key feature of human type 2 diabetes. Next, we utilised osmotic mini-pumps to infuse streptozotocin (STZ; doses ranging 80–200 mg/kg) over the course of 14-days to decrease insulin-producing capacity thus promoting hyperglycemia. Using this new approach, we demonstrate a dose-dependent effect of STZ on circulating glucose and insulin levels as well as glucose tolerance, while retaining a state of obesity. Importantly, we found that insulin secretion in response to a glucose load was present, but reduced in a dose-dependent manner by increasing STZ. In conclusion, we demonstrate a novel method that enables induction of discrete stages of type 2 diabetes in rodents that closely mirrors the different stages of type 2 diabetes in humans.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | diabetes, hyperglycaemia, diabetic model |
| Research Division: | Medical and Health Sciences |
| Research Group: | Clinical Sciences |
| Research Field: | Endocrinology |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Diabetes |
| UTAS Author: | Premilovac, D (Dr Dino Premilovac) |
| UTAS Author: | Gasperini, RJ (Dr Rob Gasperini) |
| UTAS Author: | West, A (Professor Adrian West) |
| UTAS Author: | Keske, M (Dr Michelle Keske) |
| UTAS Author: | Taylor, BV (Professor Bruce Taylor) |
| UTAS Author: | Foa, L (Professor Lisa Foa) |
| ID Code: | 122030 |
| Year Published: | 2017 |
| Funding Support: | National Health and Medical Research Council (1088952) |
| Web of Science® Times Cited: | 2 |
| Deposited By: | Medicine |
| Deposited On: | 2017-10-26 |
| Last Modified: | 2018-09-11 |
| Downloads: | 69 View Download Statistics |
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