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A New Method for Targeted and Sustained Induction of Type 2 Diabetes in Rodents

Citation

Premilovac, D and Gasperini, RJ and Sawyer, S and West, A and Keske, M and Taylor, BV and Foa, L, A New Method for Targeted and Sustained Induction of Type 2 Diabetes in Rodents, Scientific Reports, 7 Article 14158. ISSN 2045-2322 (2017) [Refereed Article]


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DOI: doi:10.1038/s41598-017-14114-4

Abstract

Type 2 diabetes is a chronic metabolic disorder that is becoming a leading cause of morbidity and mortality. The prolonged time-course of human type 2 diabetes makes modelling of the disease difficult and additional animal models and methodologies are needed. The goal of this study was to develop and characterise a new method that allows controlled, targeted and sustained induction of discrete stages of type 2 diabetes in rodents. Using adult, male rats, we employed a three-week high fat-diet regimen and confirmed development of obesity-associated glucose intolerance, a key feature of human type 2 diabetes. Next, we utilised osmotic mini-pumps to infuse streptozotocin (STZ; doses ranging 80200 mg/kg) over the course of 14-days to decrease insulin-producing capacity thus promoting hyperglycemia. Using this new approach, we demonstrate a dose-dependent effect of STZ on circulating glucose and insulin levels as well as glucose tolerance, while retaining a state of obesity. Importantly, we found that insulin secretion in response to a glucose load was present, but reduced in a dose-dependent manner by increasing STZ. In conclusion, we demonstrate a novel method that enables induction of discrete stages of type 2 diabetes in rodents that closely mirrors the different stages of type 2 diabetes in humans.

Item Details

Item Type:Refereed Article
Keywords:diabetes, hyperglycaemia, diabetic model
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Endocrinology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Diabetes
Author:Premilovac, D (Dr Dino Premilovac)
Author:Gasperini, RJ (Dr Rob Gasperini)
Author:West, A (Professor Adrian West)
Author:Keske, M (Dr Michelle Keske)
Author:Taylor, BV (Professor Bruce Taylor)
Author:Foa, L (Associate Professor Lisa Foa)
ID Code:122030
Year Published:2017
Funding Support:National Health and Medical Research Council (1088952)
Deposited By:Medicine (Discipline)
Deposited On:2017-10-26
Last Modified:2017-10-30
Downloads:0

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