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Abnormal M1/M2 macrophage phenotype profiles in the small airway wall and lumen in smokers and chronic obstructive pulmonary disease (COPD)
Citation
Eapen, MS and Hasbro, PM and McAlinden, K and Kim, RY and Ward, C and Hackett, TL and Walters, EH and Sohal, SS, Abnormal M1/M2 macrophage phenotype profiles in the small airway wall and lumen in smokers and chronic obstructive pulmonary disease (COPD), Scientific reports, 7, (1) Article 13392. ISSN 2045-2322 (2017) [Refereed Article]
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Copyright Statement
Copyright 2017 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1038/s41598-017-13888-x
Abstract
We explore potential dysregulation of macrophage phenotypes in COPD pathogenesis through integrated study of human small airway tissue, bronchoalveolar lavage (BAL) and an experimental murine model of COPD. We evaluated human airway tissue and BAL from healthy controls, normal lung function smokers (NLFS), and COPD subjects. Both small airways and BAL cells were immunohistochemically stained with anti-CD68 for total macrophages and with anti-CD163 for M2, and anti-iNOS for M1 macrophages. Multiplex ELISA measured BAL cytokines. Comparable cigarette smoke-induced experimental COPD mouse model was assessed for relevant mRNA profiles. We found an increase in pro-inflammatory M1s in the small airways of NLFS and COPD compared to controls with a reciprocal decrease in M2 macrophages, which remained unchanged among pathological groups. However, luminal macrophages showed a dominant M2 phenotype in both NLFS and COPD subjects. BAL cytokine skewed towards an M2 profile with increase in CCL22, IL-4, IL-13, and IL-10 in both NLFS and COPDs. The mouse-model of COPD showed similar increase in mRNA for M2 markers. Our finding suggests abnormal macrophage switching in both mucosal and luminal areas of COPD patients, that strongly associated with cytokine balance. There may be potential for beneficial therapeutic cytokine manipulation of macrophage phenotypes in COPD.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | COPD, inflammation |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Cardiovascular medicine and haematology |
| Research Field: | Respiratory diseases |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | Eapen, MS (Dr Mathew Eapen) |
| UTAS Author: | Walters, EH (Professor Haydn Walters) |
| UTAS Author: | Sohal, SS (Dr Sukhwinder Sohal) |
| ID Code: | 121978 |
| Year Published: | 2017 |
| Web of Science® Times Cited: | 93 |
| Deposited By: | Health Sciences |
| Deposited On: | 2017-10-24 |
| Last Modified: | 2022-08-25 |
| Downloads: | 149 View Download Statistics |
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