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Fractionation of dissolved organic matter on coupled reversed-phase monolithic columns and characterisation using reversed-phase liquid chromatography-high resolution mass spectrometry

Citation

Sandron, S and Davies, NW and Wilson, RR and Rojas Cardona, A and Haddad, PR and Nesterenko, PN and Paull, B, Fractionation of dissolved organic matter on coupled reversed-phase monolithic columns and characterisation using reversed-phase liquid chromatography-high resolution mass spectrometry, Chromatographia pp. 1-11. ISSN 0009-5893 (2017) [Refereed Article]

Copyright Statement

© Springer-Verlag Berlin Heidelberg 2017

DOI: doi:10.1007/s10337-017-3324-0

Abstract

Eleven Onyx monolithic C18 columns (100 x 3 mm) were connected in series to obtain a high-capacity reversed-phase HPLC column providing 110,000 theoretical plates. The column was used to fractionate a complex mixture of semi-polar and apolar components within marine dissolved organic matter (DOM), isolated using solid-phase extraction (SPE) on poly(styrene–divinylbenzene) cartridges, and Suwannee River natural organic matter, isolated by reverse osmosis. In each case, 15 isolated fractions were further separated using a second-dimension reversed-phase HPLC coupled with high-resolution mass spectrometry. Successful fractionation of the major compositional materials within DOM (i.e. carboxylic-rich alicyclic molecules, CRAM) in order of decreasing polarity was confirmed. Upon formulae assignment, homologous series of compounds were fractionated according to decreasing O/C ratio and increasing H/C ratio. Different compounds with the same exact masses were separated across six fractions, highlighting the immensely complex nature of the material, with the presence of potentially hundreds of molecules for each molecular formula. Both samples showed clear compositional differences not only related to source (seawater or freshwater), but also to the extraction method applied (SPE or reverse osmosis).

Item Details

Item Type:Refereed Article
Keywords:dissolved organic matter, fractionation, coupled monolithic columns, reversed-phase liquid chromatography, high-resolution mass spectrometry
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Sandron, S (Dr Sara Sandron)
Author:Davies, NW (Associate Professor Noel Davies)
Author:Wilson, RR (Dr Richard Wilson)
Author:Rojas Cardona, A (Mr Alfonso Rojas)
Author:Haddad, PR (Professor Paul Haddad)
Author:Nesterenko, PN (Professor Pavel Nesterenko)
Author:Paull, B (Professor Brett Paull)
ID Code:121903
Year Published:2017
Funding Support:Australian Research Council (DP130101518)
Deposited By:Central Science Laboratory
Deposited On:2017-10-19
Last Modified:2017-11-13
Downloads:0

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