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Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt
Citation
Chandler, JC and Fitzgibbon, QP and Smith, G and Elizur, A and Ventura, T, Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt, Developmental Biology, 430, (2) pp. 337-345. ISSN 0012-1606 (2017) [Refereed Article]
Copyright Statement
Copyright 2017 Elsevier Inc.
DOI: doi:10.1016/j.ydbio.2017.08.031
Abstract
Sex determination pathways are extensively diverse across species, with the master sex-determinants being the most variable element. Despite this, there is a family of DM-domain transcription factors (Dmrts), which hold a highly conserved function in sexual development. This work is the first to describe a heterogametic sex-linked Dmrt in an invertebrate species, the Eastern spiny lobster, Sagmariasus verreauxi. We have termed the Ylinked, truncated paralogue of the autosomal iDmrt1, Sv-iDMY. Considering the master sex-determining function of both DMY in medaka and DM-W in frog, we hypothesised a similar function of Sv-iDMY. By conducting temporal expression analyses during embryogenesis we have identified a putative male sexdetermining period during which iDMY > iDmrt1. Employing a GAL4-transactivation assay we then demonstrate the dominant negative suppression of iDMY over its autosomal iDmrt1 paralogue, suggesting the mechanism with which iDMY determines sex. Comparative analyses of Sv-iDMY, DM-W and medaka DMY, highlight the C′-mediated features of oligomerisation and transactivation as central to the mechanism that each exerts. Indeed, these features may underpin the plasticity facilitating the convergent emergence of these three sporadic sex-linked master-Dmrts.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | master sex-determinant, Dmrt, DMY, transactivation domain, Crustacea |
| Research Division: | Agricultural, Veterinary and Food Sciences |
| Research Group: | Fisheries sciences |
| Research Field: | Aquaculture |
| Objective Division: | Animal Production and Animal Primary Products |
| Objective Group: | Fisheries - aquaculture |
| Objective Field: | Fisheries - aquaculture not elsewhere classified |
| UTAS Author: | Fitzgibbon, QP (Associate Professor Quinn Fitzgibbon) |
| UTAS Author: | Smith, G (Professor Gregory Smith) |
| ID Code: | 121452 |
| Year Published: | 2017 |
| Funding Support: | Australian Research Council (DP160103320) |
| Web of Science® Times Cited: | 25 |
| Deposited By: | Sustainable Marine Research Collaboration |
| Deposited On: | 2017-09-29 |
| Last Modified: | 2018-03-20 |
| Downloads: | 0 |
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