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Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt

Citation

Chandler, JC and Fitzgibbon, QP and Smith, G and Elizur, A and Ventura, T, Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt, Developmental Biology, 430, (2) pp. 337-345. ISSN 0012-1606 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 Elsevier Inc.

DOI: doi:10.1016/j.ydbio.2017.08.031

Abstract

Sex determination pathways are extensively diverse across species, with the master sex-determinants being the most variable element. Despite this, there is a family of DM-domain transcription factors (Dmrts), which hold a highly conserved function in sexual development. This work is the first to describe a heterogametic sex-linked Dmrt in an invertebrate species, the Eastern spiny lobster, Sagmariasus verreauxi. We have termed the Y-linked, truncated paralogue of the autosomal iDmrt1, Sv-iDMY. Considering the master sex-determining function of both DMY in medaka and DM-W in frog, we hypothesised a similar function of Sv-iDMY. By conducting temporal expression analyses during embryogenesis we have identified a putative male sex-determining period during which iDMY>iDmrt1. Employing a GAL4-transactivation assay we then demonstrate the dominant negative suppression of iDMY over its autosomal iDmrt1 paralogue, suggesting the mechanism with which iDMY determines sex. Comparative analyses of Sv-iDMY, DM-W and medaka DMY, highlight the C′-mediated features of oligomerisation and transactivation as central to the mechanism that each exerts. Indeed, these features may underpin the plasticity facilitating the convergent emergence of these three sporadic sex-linked master-Dmrts.

Item Details

Item Type:Refereed Article
Keywords:lobster, sex
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Fitzgibbon, QP (Dr Quinn Fitzgibbon)
Author:Smith, G (Associate Professor Gregory Smith)
ID Code:121452
Year Published:2017
Funding Support:Australian Research Council (DP160103320)
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2017-09-29
Last Modified:2017-11-13
Downloads:0

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