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The development of a positive non-infectious control for the detection of Perkinsus using the Ray test

Citation

Moore, BR and Kleeman, SN and Lester, RJG, The development of a positive non-infectious control for the detection of Perkinsus using the Ray test, Journal of Shellfish Research, 21, (2) pp. 871-873. ISSN 0730-8000 (2002) [Refereed Article]


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Abstract

To establish a noncontagious control for the Ray thioglycollate test for the detection of Perkinsus in mollusks we evaluated nonviable stages of P. olseni for enlargement of hypnospores and blue/black iodine stain. Trophozoites made nonviable with formalin, irradiation or colchicine failed to swell in thioglycollate. They remained small and did not differentially stain in iodine. Trophozoites that had already developed into hypnospores in thioglycollate were rendered inactive by freezing, ethanol or formalin immersion. They retained their iodinophilic properties and thus could provide a partial control for the Ray Test.

Item Details

Item Type:Refereed Article
Keywords:Perkinsus, abalone, fluid thioglycollate medium
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Pests and Diseases
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Molluscs (excl. Oysters)
Author:Moore, BR (Mr Bradley Moore)
ID Code:121421
Year Published:2002
Web of Science® Times Cited:1
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2017-09-28
Last Modified:2017-10-03
Downloads:0

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