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Time-Resolved Pharmacological Studies using Automated, Online Monitoring of Five Parallel Suspension Cultures
Citation
Alhusban, A and Breadmore, MC and Gueven, N and Guijt, RM, Time-Resolved Pharmacological Studies using Automated, Online Monitoring of Five Parallel Suspension Cultures, Scientific Reports, 7 pp. 1-9. ISSN 2045-2322 (2017) [Refereed Article]
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Copyright Statement
Copyright 2017 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1038/s41598-017-10472-1
Abstract
Early stage pharmacological studies rely on in vitromethodologies for screening and testing
compounds. Conventional assays based on endpoint measurements provide limited information
because the lack in temporal resolution may not determine the pharmacological efect at its maximum.
We developed an on-line, automated system for near real-time monitoring of extracellular content
from fve parallel suspension cultures, combining cell density measurements with a high-resolution
separations every 12 minutes for 4 days. Selector and switching valves provide the fluidic control
required to sample from one culture during the analysis of the previous sample from another culture, a
time-saving measure that is fundamental to the throughput of the presented system. The system was
applied to study the metabolic efects of the drugs rotenone, β-lapachone and clioquinol using lactate
as metabolic indicator. For each drug, 96 assays were executed on the extracellular matrix at three
concentrations with two controls in parallel, consuming only 5.78mL of media from each culture over
four days, less than 60μL per analysis. The automated system provides high sample throughput, good
temporal resolution and low sample consumption combined with a rugged analytical method with
adequate sensitivity, providing a promising new platform for pharmacological and biotechnological
studies.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Pharmacology and pharmaceutical sciences |
| Research Field: | Clinical pharmacology and therapeutics |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the health sciences |
| UTAS Author: | Alhusban, A (Mr Ala Alhusban) |
| UTAS Author: | Breadmore, MC (Professor Michael Breadmore) |
| UTAS Author: | Gueven, N (Dr Nuri Guven) |
| UTAS Author: | Guijt, RM (Dr Rosanne Guijt) |
| ID Code: | 121250 |
| Year Published: | 2017 |
| Web of Science® Times Cited: | 9 |
| Deposited By: | Medicine |
| Deposited On: | 2017-09-20 |
| Last Modified: | 2018-09-11 |
| Downloads: | 126 View Download Statistics |
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