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Time-Resolved Pharmacological Studies using Automated, Online Monitoring of Five Parallel Suspension Cultures

Citation

Alhusban, A and Breadmore, MC and Gueven, N and Guijt, RM, Time-Resolved Pharmacological Studies using Automated, Online Monitoring of Five Parallel Suspension Cultures, Scientific Reports, 7 pp. 1-9. ISSN 2045-2322 (2017) [Refereed Article]


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Copyright Statement

Copyright 2017 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/s41598-017-10472-1

Abstract

Early stage pharmacological studies rely on in vitromethodologies for screening and testing compounds. Conventional assays based on endpoint measurements provide limited information because the lack in temporal resolution may not determine the pharmacological efect at its maximum. We developed an on-line, automated system for near real-time monitoring of extracellular content from fve parallel suspension cultures, combining cell density measurements with a high-resolution separations every 12 minutes for 4 days. Selector and switching valves provide the fluidic control required to sample from one culture during the analysis of the previous sample from another culture, a time-saving measure that is fundamental to the throughput of the presented system. The system was applied to study the metabolic efects of the drugs rotenone, β-lapachone and clioquinol using lactate as metabolic indicator. For each drug, 96 assays were executed on the extracellular matrix at three concentrations with two controls in parallel, consuming only 5.78mL of media from each culture over four days, less than 60μL per analysis. The automated system provides high sample throughput, good temporal resolution and low sample consumption combined with a rugged analytical method with adequate sensitivity, providing a promising new platform for pharmacological and biotechnological studies.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Clinical Pharmacology and Therapeutics
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
UTAS Author:Alhusban, A (Mr Ala Alhusban)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
UTAS Author:Gueven, N (Dr Nuri Guven)
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
ID Code:121250
Year Published:2017
Web of Science® Times Cited:1
Deposited By:Medicine
Deposited On:2017-09-20
Last Modified:2018-09-11
Downloads:68 View Download Statistics

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