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β-catenin, Twist and Snail: Transcriptional regulation of EMT in smokers and COPD, and relation to airflow obstruction
Citation
Mahmood, MQ and Walters, EH and Shukla, SD and Weston, S and Muller, HK and Ward, C and Sohal, SS, β-catenin, Twist and Snail: Transcriptional regulation of EMT in smokers and COPD, and relation to airflow obstruction, Scientific Reports, 7, (1) Article 10832. ISSN 2045-2322 (2017) [Refereed Article]
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Copyright Statement
Copyright 2017 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1038/s41598-017-11375-x
Abstract
COPD is characterised by poorly reversible airflow obstruction usually due to cigarette smoking. The transcription factor clusters of β-catenin/Snail1/Twist has been implicated in the process of epithelial mesenchymal transition (EMT), an intermediate between smoking and airway fibrosis, and indeed lung cancer. We have investigated expression of these transcription factors and their "cellular localization" in bronchoscopic airway biopsies from patients with COPD, and in smoking and non-smoking controls. An immune-histochemical study compared cellular protein expression of β-catenin, Snail1 and Twist, in these subject groups in 3 large airways compartment: epithelium (basal region), reticular basement membrane (Rbm) and underlying lamina propria (LP). β-catenin and Snail1 expression was generally high in all subjects throughout the airway wall with marked cytoplasmic to nuclear shift in COPD (P < 0.01). Twist expression was generalised in the epithelium in normal but become more basal and nuclear with smoking (P < 0.05). In addition, β-catenin and Snail1 expression, and to lesser extent of Twist, was related to airflow obstruction and to expression of a canonical EMT biomarker (S100A4). The β-catenin-Snail1-Twist transcription factor cluster is up-regulated and nuclear translocated in smokers and COPD, and their expression is closely related to both EMT activity and airway obstruction.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | COPD, lung cancer |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Cardiovascular medicine and haematology |
| Research Field: | Respiratory diseases |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Clinical health not elsewhere classified |
| UTAS Author: | Mahmood, MQ (Mr Malik Mahmood) |
| UTAS Author: | Walters, EH (Professor Haydn Walters) |
| UTAS Author: | Shukla, SD (Mr Shakti Shukla) |
| UTAS Author: | Weston, S (Mr Steven Weston) |
| UTAS Author: | Muller, HK (Professor Konrad Muller) |
| UTAS Author: | Sohal, SS (Dr Sukhwinder Sohal) |
| ID Code: | 121246 |
| Year Published: | 2017 |
| Web of Science® Times Cited: | 25 |
| Deposited By: | Health Sciences |
| Deposited On: | 2017-09-19 |
| Last Modified: | 2018-09-13 |
| Downloads: | 52 View Download Statistics |
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