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Three nuclear genes for phylogenetic, SNP and population genetic studies of molluscs and other invertebrates

Citation

Audzijonyte, A and Vrijenhoek, RC, Three nuclear genes for phylogenetic, SNP and population genetic studies of molluscs and other invertebrates, Molecular Ecology Resources, 10, (1) pp. 200-204. ISSN 1755-098X (2010) [Refereed Article]


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DOI: doi:10.1111/j.1755-0998.2009.02737.x

Abstract

The study reports new primers capable of amplifying fragments from three nuclear protein-coding genes in a variety of deep-sea molluscs and annelids adenine nucleotide translocase (Ant), calmodulin (Cal) and cyclophilin A (CycA). The Ant primers appear to be restricted to bivalve molluscs, whereas the Cal and CycA primers also amplified appropriate gene fragments from Lepetodrilus gastropod molluscs and Osedax polychaete worms. The amplified fragment of Cal contains an intron in the molluscs, but no intron was detected in the Ant and CycA fragments from any of the tested animals. DNA sequences generated by the three primer sets exhibited one to 15 single nucleotide polymorphism sites in deep-sea vesicomyid clams and Osedax boneworms. The observed levels of polymorphism indicate that the genes are likely to be useful in both population genetic and phylogenetic analyses of different invertebrate taxa.

Item Details

Item Type:Refereed Article
Keywords:adenine nucleotide translocase, calmodulin, cyclophilin A, exons, introns, single copy nuclear genes
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Marine Flora, Fauna and Biodiversity
Author:Audzijonyte, A (Dr Asta Audzijonyte)
ID Code:121149
Year Published:2010
Web of Science® Times Cited:10
Deposited By:Centre for Ecology and Biodiversity
Deposited On:2017-09-12
Last Modified:2017-09-13
Downloads:0

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