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Detection of paralytic shellfish toxins in mussels and oysters using the qualitative neogen lateral-flow immunoassay: an interlaboratory study

Citation

Dorantes Aranda, JJ and Tan, JYC and Hallegraeff, GM and Campbell, K and Ugalde, SC and Harwood, DT and Bartlett, JK and Campas, M and Crooks, S and Gerssen, A and Harrison, K and Huet, A-C and Jordan, TB and Koeberl, M and Monaghan, T and Murray, S and Nimmagadda, R and Ooms, C and Quinlan, RK and Shi, F and Turner, AD and Yakes, BJ and Turnbull, AR, Detection of paralytic shellfish toxins in mussels and oysters using the qualitative neogen lateral-flow immunoassay: an interlaboratory study, Journal of AOAC International, 101, (2) Article 170221. ISSN 1060-3271 (2017) [Refereed Article]


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DOI: doi:10.5740/jaoacint.17-0221

Abstract

Paralytic shellfish toxins (PSTs) in bivalve molluscs represent a public health risk and are controlled via compliance with a regulatory limit of 0.8 mg saxitoxin (STX)⋅2HCl equivalents per kilogram of shellfish meat (eq/kg). Shellfish industries would benefit from the use of rapid immunological screening tests for PSTs to be used for regulation, but to date none have been fully validated. An interlaboratory study involving 16 laboratories was performed to determine the suitability of the Neogen test to detect PSTs in mussels and oysters. Participants performed the standard protocol recommended by the manufacturer and a modified protocol with a conversion step to improve detection of gonyautoxin 1&4. The statistical analysis showed that the protocols had good homogeneity across all laboratories, with satisfactory repeatability, laboratory, and reproducibility variation near the regulatory level. The mean probability of detection (POD) at 0.8 mg STX⋅2HCl eq/kg using the standard protocol in mussels and oysters was 0.966 and 0.997, respectively, and 0.968 and 0.966 using the modified protocol. The estimated LOD in mussels was 0.316 mg STX⋅2HCl eq/kg with the standard and 0.682 mg STX⋅2HCl eq/kg with the modified protocol, and 0.710 and 0.734 mg STX⋅2HCl eq/kg for oysters, respectively. The Neogen test may be acceptable for regulatory purposes for oysters in accordance with European Commission directives in which the standard protocol provides, at the regulatory level, a probability of a negative response of 0.033 on 95% of occasions. Its use for mussels is less consistent at the regulatory level due to the wide prediction interval around the POD.

Item Details

Item Type:Refereed Article
Keywords:international lab validation
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Dorantes Aranda, JJ (Dr Juan Dorantes Aranda)
Author:Hallegraeff, GM (Professor Gustaaf Hallegraeff)
Author:Ugalde, SC (Ms Sarah Ugalde)
Author:Quinlan, RK (Ms Rae Quinlan)
ID Code:121094
Year Published:2017
Deposited By:Centre for Ecology and Biodiversity
Deposited On:2017-09-08
Last Modified:2017-09-18
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