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The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels

Citation

Hwang, E-S and Cao, C and Hong, S and Jung, H-J and Cha, C-Y and Choi, J-B and Kim, Y-J and Baik, S, The DNA hybridization assay using single-walled carbon nanotubes as ultrasensitive, long-term optical labels, Nanotechnology, 17, (14) pp. 3442-3445. ISSN 0957-4484 (2006) [Refereed Article]

Copyright Statement

Copyright 2006 IOP Publishing Ltd.

DOI: doi:10.1088/0957-4484/17/14/016

Abstract

Single walled carbon nanotubes (SWNTs) exhibit strong Raman signals as well as fluorescence emissions in the near infrared region. Such signals do not blink or photobleach under prolonged excitation, which is an advantage in optical nano-biomarker applications. In this paper, we present single-stranded DNA conjugated SWNT probes to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblasts and Escherichia coli are used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrates excellent sensitivity and specificity of the probes. The results show that SWNTs may be used as generic nano-biomarkers for the precise detection of specific kinds of genes.

Item Details

Item Type:Refereed Article
Keywords:carbon nanotube, DNA hybridization
Research Division:Technology
Research Group:Nanotechnology
Research Field:Nanobiotechnology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Hong, S (Dr Sanghyun Hong)
ID Code:120959
Year Published:2006
Web of Science® Times Cited:27
Deposited By:Plant Science
Deposited On:2017-09-04
Last Modified:2017-11-14
Downloads:0

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