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Revealing the roles of GORK channels and NADPH oxidase in acclimation to hypoxia in Arabidopsis

Citation

Wang, F and Chen, Z-H and Liu, X and Colmer, TD and Shabala, L and Salih, A and Zhou, M and Shabala, S, Revealing the roles of GORK channels and NADPH oxidase in acclimation to hypoxia in Arabidopsis, Journal of Experimental Botany, 68, (12) pp. 3191-3204. ISSN 0022-0957 (2017) [Refereed Article]


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Copyright Statement

The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. Licensed under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/

DOI: doi:10.1093/jxb/erw378

Abstract

Regulation of root cell K+ is essential for acclimation to low oxygen stress. The potential roles of GORK (depolarization-activated guard cell outward-rectifying potassium) channels and RBOHD (respiratory burst oxidase homologue D) in plant adaptive responses to hypoxia were investigated in the context of tissue specificity (epidermis versus stele; elongation versus mature zone) in roots of Arabidopsis. The expression of GORK and RBOHD was down-regulated by 2- to 3-fold within 1 h and 24 h of hypoxia treatment in Arabidopsis wild-type (WT) roots. Interestingly, a loss of the functional GORK channel resulted in a waterlogging-tolerant phenotype, while rbohD knockout was sensitive to waterlogging. To understand their functions under hypoxia stress, we studied K+, Ca2+, and reactive oxygen species (ROS) distribution in various root cell types. gork1-1 plants had better K+ retention ability in both the elongation and mature zone compared with the WT and rbohD under hypoxia. Hypoxia induced a Ca2+ increase in each cell type after 72 h, and the increase was much less pronounced in rbohD than in the WT. In most tissues except the elongation zone in rbohD, the H2O2 concentration had decreased after 1 h of hypoxia, but then increased significantly after 24 h of hypoxia in each zone and tissue, further suggesting that RBOHD may shape hypoxia-specific Ca2+ signatures via the modulation of apoplastic H2O2 production. Taken together, our data suggest that plants lacking functional GORK channels are more capable of retaining K+ for their better performance under hypoxia, and that RBOHD is crucial in hypoxia-induced Ca2+ signalling for stress sensing and acclimation mechanism.

Item Details

Item Type:Refereed Article
Keywords:hypoxia, ROS, potassium, flooding, calcium, epidermis, fluorescence dyes, homeostasis, reactive oxygen species, signalling, stele, superoxide
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
UTAS Author:Wang, F (Miss Feifei Wang)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Zhou, M (Professor Meixue Zhou)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:120875
Year Published:2017
Web of Science® Times Cited:18
Deposited By:Agriculture and Food Systems
Deposited On:2017-08-31
Last Modified:2018-07-27
Downloads:52 View Download Statistics

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