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Expression of the proangiogenic protein hepatoma-derived growth factor in neovessels in an arterial venous loop-based tissue engineering chamber

Citation

Chan, EC and Tai, MH and Wu, PC and Kuo, HM and Jiang, F and Tee, R and Morrison, WA and Dusting, GJ and Liu, GS, Expression of the proangiogenic protein hepatoma-derived growth factor in neovessels in an arterial venous loop-based tissue engineering chamber, Biomedical Engineering: Applications, Basis and Communications, 25, (5) Article 1340007. ISSN 1793-7132 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 World Scientific Publishing Company

DOI: doi:10.4015/S1016237213400073

Abstract

Boosting angiogenesis is a crucial process to enhance tissue growth in tissue engineering (TE). Hepatoma-derived growth factor (HDGF) has been identified as an angiogenic factor, but its involvement in angiogenesis in an arteriovenous loop-based TE chamber developed by the laboratory is unclear. In this study, the authors first examined the effects of HDGF on angiogenic responses in endothelial cells and in a corneal model of neovascularization, and then characterized the expression of HDGF in the TE chamber. HDGF (1-500 ng/mL) induced concentration-dependent angiogenic responses in human endothelial cells in vitro (proliferation, migration, and tube formation). Local application of HDGF stimulated neovascularization in a rat model of corneal angiogenesis. In the TE chamber, there was an increase in blood vessel volume from day 3 to day 14. Immunofluorescence microscopy revealed that HDGF is highly expressed in the neovessels in the chamber. Peak expression of HDGF (day 3) coincided with the infiltration of inflammatory cells, and the mRNA level of endogenous HDGF correlated with that of tumor necrosis factor α (TNFα). In vitro, TNFα stimulated HDGF expression in endothelial cells. The data suggest that HDGF may be involved in angiogenic responses in the TE chamber and the proinflammatory cytokine TNFα may have a pivotal role in stimulating HDGF expression. Enhancing HDGF signaling may be a new approach to extend vascularization for TE. © 2013 National Taiwan University.

Item Details

Item Type:Refereed Article
Keywords:angiogenesis, hepatoma-derived growth factor, inflammation, tissue engineering, tumor necrosis factor 
Research Division:Technology
Research Group:Medical Biotechnology
Research Field:Gene and Molecular Therapy
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Inherited Diseases (incl. Gene Therapy)
Author:Liu, GS (Dr Guei-Sheung Liu)
ID Code:120872
Year Published:2013
Deposited By:Menzies Institute for Medical Research
Deposited On:2017-08-31
Last Modified:2017-09-15
Downloads:0

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