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Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species


Kiani-Pouya, A and Roessner, U and Jayasinghe, NS and Lutz, A and Rupasinghe, T and Bazihizina, N and Bohm, J and Alharbi, S and Hedrich, R and Shabala, S, Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species, Plant Cell and Environment, 40, (9) pp. 1900-1915. ISSN 0140-7791 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 John Wiley and Sons Ltd

DOI: doi:10.1111/pce.12995


Epidermal bladder cells (EBCs) have been postulated to assist halophytes in coping with saline environments. However, little direct supporting evidence is available. Here, Chenopodium quinoa plants were grown under saline conditions for 5 weeks. One day prior to salinity treatment, EBCs from all leaves and petioles were gently removed by using a soft cosmetic brush and physiological, ionic and metabolic changes in brushed and non-brushed leaves were compared. Gentle removal of EBC neither initiated wound metabolism nor affected the physiology and biochemistry of control-grown plants but did have a pronounced effect on salt-grown plants, resulting in a salt-sensitive phenotype. Of 91 detected metabolites, more than half were significantly affected by salinity. Removal of EBC dramatically modified these metabolic changes, with the biggest differences reported for gamma-aminobutyric acid (GABA), proline, sucrose and inositol, affecting ion transport across cellular membranes (as shown in electrophysiological experiments). This work provides the first direct evidence for a role of EBC in salt tolerance in halophytes and attributes this to (1) a key role of EBC as a salt dump for external sequestration of sodium; (2) improved K+ retention in leaf mesophyll and (3) EBC as a storage space for several metabolites known to modulate plant ionic relations.

Item Details

Item Type:Refereed Article
Keywords:halophyte, sodium, salinity, stress, epidermal bladder cells, metabolic profile, sodium sequestration
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Kiani-Pouya, A (Dr Ali Kiani-Pouya)
UTAS Author:Bazihizina, N (Dr Nadia Bazihizina)
UTAS Author:Bohm, J (Ms Jennifer Bohm)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:120867
Year Published:2017
Web of Science® Times Cited:62
Deposited By:Agriculture and Food Systems
Deposited On:2017-08-31
Last Modified:2018-06-01

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