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Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway
Citation
Kung, ML and Tsai, HE and Hu, TS and Kuo, HM and Liu, LF and Chen, SC and Lin, PR and Ma, YL and Wang, EM and Liu, GS and Liu, JK and Tai, MH, Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway, Biochemical and Biophysical Research Communications, 425, (2) pp. 169-176. ISSN 1090-2104 (2012) [Refereed Article]
Copyright Statement
copyright 2012 Elsevier
DOI: doi:10.1016/j.bbrc.2012.07.060
Abstract
Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Cell migration, Hepatoma-derived growth factor, Podosome, PI3K, Akt |
| Research Division: | Technology |
| Research Group: | Medical Biotechnology |
| Research Field: | Gene and Molecular Therapy |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Inherited Diseases (incl. Gene Therapy) |
| Author: | Liu, GS (Dr Guei-Sheung Liu) |
| ID Code: | 120837 |
| Year Published: | 2012 |
| Web of Science® Times Cited: | 13 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2017-08-31 |
| Last Modified: | 2017-11-06 |
| Downloads: | 0 |
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