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Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway
journal contribution
posted on 2023-05-19, 10:49 authored by Kung, ML, Tsai, HE, Hu, TS, Kuo, HM, Liu, LF, Chen, SC, Lin, PR, Ma, YL, Wang, EM, Guei-Sheung LiuGuei-Sheung Liu, Liu, JK, Tai, MHHepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration.
History
Publication title
Biochemical and Biophysical Research CommunicationsVolume
425Pagination
169-176ISSN
1090-2104Department/School
Menzies Institute for Medical ResearchPublisher
Elsevier Inc.Place of publication
United StatesRights statement
copyright 2012 ElsevierRepository Status
- Restricted