Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration.

Item Type:	Refereed Article
Keywords:	Cell migration, Hepatoma-derived growth factor, Podosome, PI3K, Akt
Research Division:	Biomedical and Clinical Sciences
Research Group:	Medical biotechnology
Research Field:	Gene and molecular therapy
Objective Division:	Health
Objective Group:	Clinical health
Objective Field:	Clinical health not elsewhere classified
UTAS Author:	Liu, GS (Associate Professor Guei-Sheung Liu)
ID Code:	120837
Year Published:	2012
Web of Science® Times Cited:	18
Deposited By:	Menzies Institute for Medical Research
Deposited On:	2017-08-31
Last Modified:	2017-11-06
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