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Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway

Citation

Kung, ML and Tsai, HE and Hu, TS and Kuo, HM and Liu, LF and Chen, SC and Lin, PR and Ma, YL and Wang, EM and Liu, GS and Liu, JK and Tai, MH, Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway, Biochemical and Biophysical Research Communications, 425, (2) pp. 169-176. ISSN 1090-2104 (2012) [Refereed Article]

Copyright Statement

copyright 2012 Elsevier

DOI: doi:10.1016/j.bbrc.2012.07.060

Abstract

Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration.

Item Details

Item Type:Refereed Article
Keywords:Cell migration, Hepatoma-derived growth factor, Podosome, PI3K, Akt
Research Division:Technology
Research Group:Medical Biotechnology
Research Field:Gene and Molecular Therapy
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Inherited Diseases (incl. Gene Therapy)
Author:Liu, GS (Dr Guei-Sheung Liu)
ID Code:120837
Year Published:2012
Web of Science® Times Cited:13
Deposited By:Menzies Institute for Medical Research
Deposited On:2017-08-31
Last Modified:2017-11-06
Downloads:0

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