Collagen is one component of the extracellular matrix that has been widely used for constructive remodeling to facilitate cell growth and differentiation. The 3-D distribution and growth of cells within the porous scaffold suggest a clinical significance for nerve tissue engineering. In the current study, we investigated proliferation and differentiation of neuron cancer stem cells (NCSCs) on a 3-D porous collagen scaffold that mimics the natural extracellular matrix. We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold. We found that proliferation of GFP-NCSCs increased, and a single cell mass rapidly grew with unrestricted expansion between days 3 and 9 in culture. Moreover, immunostaining with neuronal nuclei (NeuN) revealed that NCSCs grown on the 3-D collagen scaffold significantly enhanced neurite outgrowth. Our findings confirmed that the 80 μm porous collagen scaffold could enhance attachment, viability and differentiation of the cancer neural stem cells. This result could provide a new application for nerve tissue engineering and nerve regeneration.

Item Type:	Refereed Article
Keywords:	Collagen scaffold, Neurite outgrowth, Neuron cancer stem cells (NCSCs)
Research Division:	Technology
Research Group:	Medical Biotechnology
Research Field:	Gene and Molecular Therapy
Objective Division:	Health
Objective Group:	Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:	Inherited Diseases (incl. Gene Therapy)
Author:	Liu, GS (Dr Guei-Sheung Liu)
ID Code:	120835
Year Published:	2012
Web of Science® Times Cited:	4
Deposited By:	Menzies Institute for Medical Research
Deposited On:	2017-08-31
Last Modified:	2017-11-06
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