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Enhancement of neurite outgrowth in neuron cancer stem cells on 3-D collagen scaffolds
Citation
Chen, CH and Kuo, SM and Liu, GS and Chen, WNU and Chuang, CW and Liu, LF, Enhancement of neurite outgrowth in neuron cancer stem cells on 3-D collagen scaffolds, Biochemical and Biophysical Research Communications, 428, (1) pp. 68-73. ISSN 1090-2104 (2012) [Refereed Article]
Copyright Statement
Copyright 2012 Elsevier Inc
DOI: doi:10.1016/j.bbrc.2012.10.007
Abstract
Collagen is one component of the extracellular matrix that has been widely used for constructive remodeling to facilitate cell growth and differentiation. The 3-D distribution and growth of cells within the porous scaffold suggest a clinical significance for nerve tissue engineering. In the current study, we investigated proliferation and differentiation of neuron cancer stem cells (NCSCs) on a 3-D porous collagen scaffold that mimics the natural extracellular matrix. We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold. We found that proliferation of GFP-NCSCs increased, and a single cell mass rapidly grew with unrestricted expansion between days 3 and 9 in culture. Moreover, immunostaining with neuronal nuclei (NeuN) revealed that NCSCs grown on the 3-D collagen scaffold significantly enhanced neurite outgrowth. Our findings confirmed that the 80 μm porous collagen scaffold could enhance attachment, viability and differentiation of the cancer neural stem cells. This result could provide a new application for nerve tissue engineering and nerve regeneration.
Item Details
Item Type: | Refereed Article |
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Keywords: | Collagen scaffold, Neurite outgrowth, Neuron cancer stem cells (NCSCs) |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Medical biotechnology |
Research Field: | Gene and molecular therapy |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Liu, GS (Associate Professor Guei-Sheung Liu) |
ID Code: | 120835 |
Year Published: | 2012 |
Web of Science® Times Cited: | 7 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2017-08-31 |
Last Modified: | 2018-04-26 |
Downloads: | 0 |
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