eCite Digital Repository

The IMPROVE Guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments)


Percie du Sert, N and Alfieri, A and Allan, SM and Carswell, HV and Deuchar, GA and Farr, TD and Flecknell, P and Gallagher, L and Gibson, CL and Haley, MJ and Macleod, MR and McColl, BW and McCabe, C and Morancho, A and Moon, LD and O'Neill, MJ and Pe'rez de Puig, I and Planas, A and Ragan, CI and Rosell, A and Roy, LA and Ryder, KO and Simats, A and Sena, ES and Sutherland, BA and Tricklebank, MD and Trueman, RC and Whitfield, L and Wong, R and Macrae, IM, The IMPROVE Guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments), Journal of Cerebral Blood Flow and Metabolism, 37, (11) pp. 3488-3517. ISSN 0271-678X (2017) [Refereed Article]


Copyright Statement

Copyright 2017 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1177/0271678X17709185


Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information).

Item Details

Item Type:Refereed Article
Keywords:improve, animal models, stroke, ischaemia, brain
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Central nervous system
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Sutherland, BA (Associate Professor Brad Sutherland)
ID Code:120820
Year Published:2017
Web of Science® Times Cited:67
Deposited By:Medicine
Deposited On:2017-08-30
Last Modified:2022-08-23
Downloads:108 View Download Statistics

Repository Staff Only: item control page