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Alzheimer’s disease cerebrospinal fluid biomarkers are not influenced by gravity drip or aspiration extraction methodology


Rembach, A and Evered, LA and Li, QX and Nash, T and Vidaurre, L and Fowler, CJ and Pertile, KK and Rumble, RL and Trounson, BO and Maher, S and Mooney, F and Farrow, M and Taddei, K and Rainey-Smith, S and Laws, SM and Macaulay, SL and Wilson, W and Darby, DG and Martins, RN and Ames, D and Collins, S and Silbert, B and Masters, CL and Doecke, JD, AIBL Research Group, Alzheimer's disease cerebrospinal fluid biomarkers are not influenced by gravity drip or aspiration extraction methodology, Alzheimer's research & therapy, 7, (1) pp. 1-8. ISSN 1758-9193 (2015) [Refereed Article]

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© 2015 Rembach et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1186/s13195-015-0157-7


Introduction: Cerebrospinal fluid (CSF) biomarkers, although of established utility in the diagnostic evaluation of Alzheimer’s disease (AD), are known to be sensitive to variation based on pre-analytical sample processing. We assessed whether gravity droplet collection versus syringe aspiration was another factor influencing CSF biomarker analyte concentrations and reproducibility.

Methods: Standardized lumbar puncture using small calibre atraumatic spinal needles and CSF collection using gravity fed collection followed by syringe aspirated extraction was performed in a sample of elderly individuals participating in a large long-term observational research trial. Analyte assay concentrations were compared.

Results: For the 44 total paired samples of gravity collection and aspiration, reproducibility was high for biomarker CSF analyte assay concentrations (concordance correlation [95%CI]: beta-amyloid1-42 (Aβ42) 0.83 [0.71 - 0.90]), t-tau 0.99 [0.98 - 0.99], and phosphorylated tau (p-tau) 0.82 [95 % CI 0.71 - 0.89]) and Bonferroni corrected paired sample t-tests showed no significant differences (group means (SD): Aβ42 366.5 (86.8) vs 354.3 (82.6), p = 0.10; t-tau 83.9 (46.6) vs 84.7 (47.4) p = 0.49; p-tau 43.5 (22.8) vs 40.0 (17.7), p = 0.05). The mean duration of collection was 10.9 minutes for gravity collection and <1 minute for aspiration.

Conclusions: Our results demonstrate that aspiration of CSF is comparable to gravity droplet collection for AD biomarker analyses but could considerably accelerate throughput and improve the procedural tolerability for assessment of CSF biomarkers.

Item Details

Item Type:Refereed Article
Keywords:Alzheimer's disease; biomarkers; cerebrospinal fluid collection
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurology and neuromuscular diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
ID Code:120734
Year Published:2015
Web of Science® Times Cited:14
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2017-08-30
Last Modified:2017-10-03
Downloads:131 View Download Statistics

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