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The toll-like receptor ligands Hiltonol® (polyICLC) and imiquimod effectively activate antigen-specific immune responses in Tasmanian devils (Sarcophilus harrisii)
Citation
Patchett, AL and Tovar, C and Corcoran, LM and Lyons, AB and Woods, GM, The toll-like receptor ligands Hiltonol® (polyICLC) and imiquimod effectively activate antigen-specific immune responses in Tasmanian devils (Sarcophilus harrisii), Developmental and Comparative Immunology, 76 pp. 352-360. ISSN 0145-305X (2017) [Refereed Article]
Copyright Statement
Copyright 2017 Elsevier Ltd.
DOI: doi:10.1016/j.dci.2017.07.004
Abstract
Devil facial tumour disease (DFTD) describes two genetically distinct transmissible tumours that pose a significant threat to the survival of the Tasmanian devil. A prophylactic vaccine could protect devils from DFTD transmission. For this vaccine to be effective, potent immune adjuvants will be required. Toll-like receptors (TLRs) promote robust immune responses in human cancer studies and are highly conserved across mammalian species. In this study, we investigated the proficiency of TLR ligands for immune activation in the Tasmanian devil using in vitro mononuclear cell stimulations and in vivo immunisation trials with a model antigen. We identified two such TLR ligands, polyICLC (Hiltonol®) (TLR3) and imiquimod (TLR7), that in combination induced significant IFNγ production from Tasmanian devil lymphocytes in vitro. Immunisation with these ligands and the model antigen keyhole limpet haemocyanin activated robust antigen-specific primary, secondary and long-term memory IgG responses. Our results support the conserved nature of TLR signaling across mammalian species. PolyICLC and imiquimod will be trialed as immune adjuvants in future DFTD vaccine formulations.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | devil facial tumor disease, imiquimod, poly-ICLC, Tasmanian devil, toll-like receptor, vaccine adjuvant |
| Research Division: | Biological Sciences |
| Research Group: | Zoology |
| Research Field: | Animal Immunology |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Immune System and Allergy |
| UTAS Author: | Patchett, AL (Dr Amanda Patchett) |
| UTAS Author: | Tovar, C (Dr Cesar Tovar Lopez) |
| UTAS Author: | Lyons, AB (Associate Professor Bruce Lyons) |
| UTAS Author: | Woods, GM (Professor Gregory Woods) |
| ID Code: | 120391 |
| Year Published: | 2017 |
| Web of Science® Times Cited: | 7 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2017-08-23 |
| Last Modified: | 2018-06-21 |
| Downloads: | 0 |
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