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The toll-like receptor ligands Hiltonol® (polyICLC) and imiquimod effectively activate antigen-specific immune responses in Tasmanian devils (Sarcophilus harrisii)

Citation

Patchett, AL and Tovar, C and Corcoran, LM and Lyons, AB and Woods, GM, The toll-like receptor ligands Hiltonol® (polyICLC) and imiquimod effectively activate antigen-specific immune responses in Tasmanian devils (Sarcophilus harrisii), Developmental and Comparative Immunology, 76 pp. 352-360. ISSN 0145-305X (2017) [Refereed Article]

Copyright Statement

Copyright 2017 Elsevier Ltd.

DOI: doi:10.1016/j.dci.2017.07.004

Abstract

Devil facial tumour disease (DFTD) describes two genetically distinct transmissible tumours that pose a significant threat to the survival of the Tasmanian devil. A prophylactic vaccine could protect devils from DFTD transmission. For this vaccine to be effective, potent immune adjuvants will be required. Toll-like receptors (TLRs) promote robust immune responses in human cancer studies and are highly conserved across mammalian species. In this study, we investigated the proficiency of TLR ligands for immune activation in the Tasmanian devil using in vitro mononuclear cell stimulations and in vivo immunisation trials with a model antigen. We identified two such TLR ligands, polyICLC (Hiltonol) (TLR3) and imiquimod (TLR7), that in combination induced significant IFNγ production from Tasmanian devil lymphocytes in vitro. Immunisation with these ligands and the model antigen keyhole limpet haemocyanin activated robust antigen-specific primary, secondary and long-term memory IgG responses. Our results support the conserved nature of TLR signaling across mammalian species. PolyICLC and imiquimod will be trialed as immune adjuvants in future DFTD vaccine formulations.

Item Details

Item Type:Refereed Article
Keywords:devil facial tumor disease, imiquimod, poly-ICLC, Tasmanian devil, toll-like receptor, vaccine adjuvant
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal Immunology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
Author:Patchett, AL (Miss Amanda Patchett)
Author:Tovar, C (Dr Cesar Tovar Lopez)
Author:Lyons, AB (Dr Bruce Lyons)
Author:Woods, GM (Professor Gregory Woods)
ID Code:120391
Year Published:2017
Web of Science® Times Cited:1
Deposited By:Menzies Institute for Medical Research
Deposited On:2017-08-23
Last Modified:2018-06-21
Downloads:0

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