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TNFα deficiency results in increased IL-1β in an early onset of spontaneous murine colitis

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De Santis, S and Kunde, D and Galleggiante, V and Liso, M and Scandiffio, L and Serino, G and Pinto, A and Campiglia, P and Sorrentino, R and Cavalcanti, E and Santino, A and Caruso, ML and Eri, R and Chieppa, M, TNFα deficiency results in increased IL-1β in an early onset of spontaneous murine colitis, Cell Death and Disease, 8, (8) pp. 1-7. ISSN 2041-4889 (2017) [Refereed Article]


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Copyright The Author(s) 2017. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/cddis.2017.397

Abstract

Inflammatory bowel disease (Crohn’s disease (CD) and ulcerative colitis (UC)) is a multifactorial disease resulting from immune dysregulation in the gut. The underlying colitis is characterized by high levels of inflammatory cytokines, including TNFα. Biological intervention for IBD patients using anti-TNFα antibodies is often an effective therapeutic solution. However, TNFα neutralization fails to induce remission in a subgroup of IBD patients, primarily in UC patients. There is a dearth of suitable animal models representing TNFα non-responders. Here we have combined one of the best UC models currently available, namely Winnie and the TNFαKO mouse to generate a TNFα-deficient Winnie to study early onset colitis. The induced TNFα deficiency with underlying colitis does not influence general health (viability and body weight) or clinical parameters (colon weight, colon length and histological colitis) when compared with the Winnie genotype alone. The molecular characterization resulted in identification of Il1β as the major elevated cytokine during early phases of colitis. Further, in vitro functional assay using bone marrow-derived dendritic cells confirmed IL-1β as the major cytokine released in the absence of TNFα. This study has generated a successful model of colitis that remains TNFα non-responsive and has demonstrated that IL-1β expression is a major pathway for the progression of colitis in this system. These data also suggest that IL-1β can be a potential target for clinical intervention of UC patients who fail to respond to TNFα neutralization.

Item Details

Item Type:Refereed Article
Keywords:ulcerative colitis, TNF, mouse model
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Innate Immunity
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Digestive System Disorders
Author:Kunde, D (Dr Dale Kunde)
Author:Eri, R (Dr Raj Eri)
ID Code:120265
Year Published:2017
Deposited By:Health Sciences
Deposited On:2017-08-17
Last Modified:2017-09-25
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