eCite Digital Repository

Plasmonic nanohole arrays for monitoring growth of bacteria and antibiotic susceptibility test


Kee, JS and Lim, SY and Perera, AP and Zhang, Y and Park, MK, Plasmonic nanohole arrays for monitoring growth of bacteria and antibiotic susceptibility test, Sensors and Actuators - B: Chemical, 182 pp. 576-583. ISSN 0925-4005 (2013) [Refereed Article]

Copyright Statement

2012 Elsevier B.V.

DOI: doi:10.1016/j.snb.2013.03.053


Rapid and sensitive detections of the growth of bacteria are essential assay procedures in the food industry, water and environment control and clinical diagnosis. Here, we present a plasmonic nanohole sensor for rapid, sensitive and quantitative monitoring of the bacterial growth and antibiotic susceptibility test. The concept is based on extraordinary optical transmission (EOT) phenomenon in plasmonic nanohole and employs Escherichia coli specific antibody for bacteria capturing. The challenge lies in detecting the much larger dimension of E. coli with the smaller penetration depths of the electromagnetic field. The plasmonic nanohole array was fabricated using mask-based deep ultraviolet (DUV) lithography method and characterized for bulk refractive index sensitivity and surface mass sensitivity. After gold-specific surface modification and antibody immobilization, bacteria were effectively captured and grown on the sensor surface. The sensor platform with temperature-controlled environment was successfully tested for monitoring the growth of E. coli. The plasmonic nanohole sensor has immense potential for clinical diagnosis such as rapid determination of pathogen susceptibility test; with bulk refractive index sensitivity of 406.4nm/RIU and surface mass sensitivity of ~1.802ng/mm2. We explored the capability of the sensor to provide sensitive monitoring of low bacterial quantity (<100cells) and rapid detection within 2h. Finally, the rapid antibiotic susceptibility test (AST) of E. coli was demonstrated with the plasmonic nanohole sensor.

Item Details

Item Type:Refereed Article
Keywords:plasmonic nanohole arrays antibiotic susceptibility test
Research Division:Biomedical and Clinical Sciences
Research Group:Medical microbiology
Research Field:Medical bacteriology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Perera, AP (Mrs Agampodi Perera)
ID Code:120261
Year Published:2013
Web of Science® Times Cited:30
Deposited By:Ecology and Biodiversity
Deposited On:2017-08-17
Last Modified:2017-09-18

Repository Staff Only: item control page