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Quantitative assessment of the functional plasticity of memory CD8(+) T cells


Baz, A and Groves, P and Buttigieg, K and Apte, SH and Kienzle, N and Kelso, A, Quantitative assessment of the functional plasticity of memory CD8(+) T cells, European Journal of Immunology, 46, (4) pp. 863-873. ISSN 0014-2980 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/eji.201545726


While the functional plasticity of memory CD4(+) T cells has been studied extensively, less is known about this property in memory CD8(+) T cells. Here, we report the direct measurement of plasticity by paired daughter analysis of effector and memory OT-I CD8(+) T cells primed in vivo with ovalbumin. Na´ve, effector, and memory OT-I cells were isolated and activated in single-cell culture; then, after the first division, their daughter cells were transferred to new cultures with and without IL-4; expression of IFN-γ and IL-4 mRNAs was measured 5 days later in the resultant subclones. Approximately 40% of clonogenic memory CD8(+) T cells were bipotential in this assay, giving rise to an IL-4(-) subclone in the absence of IL-4 and an IL-4(+) subclone in the presence of IL-4. The frequency of bipotential cells was lower among memory cells than na´ve cells but markedly higher than among 8-day effectors. Separation based on high or low expression of CD62L, CD122, CD127, or Ly6C did not identify a phenotypic marker of the bipotential cells. Functional plasticity in memory CD8(+) T-cell populations can therefore reflect modulation at the level of a single memory cell and its progeny.

Item Details

Item Type:Refereed Article
Keywords:CD8+ T cells, IFN-γ, IL-4, memory, plasticity
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies)
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Buttigieg, K (Ms Kathy Buttigieg)
ID Code:120129
Year Published:2016
Web of Science® Times Cited:1
Deposited By:Menzies Institute for Medical Research
Deposited On:2017-08-10
Last Modified:2017-09-18

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