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Analysis of methanotroph community structure using a pmoA-based microarray


Abell, GCJ and Stralis-Pavese, N and Pan, Y and Bodrossy, L, Analysis of methanotroph community structure using a pmoA-based microarray, Methods in Molecular Biology, 1096 pp. 111-122. ISSN 1064-3745 (2014) [Refereed Article]

DOI: doi:10.1007/978-1-62703-712-9-9


The analysis of methanotroph community composition is relevant to studies of methane oxidation in a number of environments where methane is a significant carbon source. The development and application of a microarray targeting the particulate methane monooxygenase gene (pmoA) have allowed a high-throughput, semiquantitative analysis of the major methanotroph groups in a number of different environments. Here we describe the use of a pmoA-based short oligo array for the analysis of methanotroph populations in sediment samples. The method is suitable for analysis of any type of environmental sample from which DNA can be extracted.

Item Details

Item Type:Refereed Article
Keywords:hybridization, methanotroph, microarray, pmoA, bacterial DNA, methane, methane monooxygenase
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Gene expression (incl. microarray and other genome-wide approaches)
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Bodrossy, L (Dr Levente Bodrossy)
ID Code:120066
Year Published:2014
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-08-09
Last Modified:2017-10-30

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