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Comparative sensitivity of commercially available aPTT reagents to mulga snake (Pseudechis australis) venom


Lincz, LF and Scorgie, FE and Johnston, CI and O'Leary, M and Prasad, R and Seldon, M and Favaloro, E and Isbister, GK, Comparative sensitivity of commercially available aPTT reagents to mulga snake (Pseudechis australis) venom, Pathology, 46, (5) pp. 444-449. ISSN 0031-3025 (2014) [Refereed Article]

DOI: doi:10.1097/PAT.0000000000000120


This study aimed to determine the relative sensitivity of activated partial thromboplastin time (aPTT) reagents to the anticoagulant effects of phospholipases inmulga snake (Pseus) venom. Twenty-one haematology laboratories participating in the Royal College of Pathologists of Australasia Quality Assurance Programs were sent human plasma samples spiked with mulga venom (n=25 total results). Results for 17 patients with mulga snake envenoming were available through the Australian Snakebite Project. Only 12 of 25 venom spiked samples returned an abnormally prolonged aPTT. Tests performed with Dade Actin FS (n=7) did not identify any of the spiked samples as abnormal. Although clotting times were significantly prolonged using the lupus anticoagulant sensitive Actin FSL (n=5, p=0.043), only one was reported as abnormal. Only laboratories using TriniCLOTaPTT S (n=6), HemosIL APTT SP (n=2) and Stago PTT-A (n=1) consistently recorded the spiked sample as being above the upp er normal reference interval. Abnormally prolonged aPTTs were recorded for four of eight patients whose tests were performed with Actin FSL, five of eight patients with TriniCLOT aPTT HS, and three of three patients using TriniCLOT aPTT S. We conclude that some reagents used for routine aPTT testing are relatively insensitive to the anticoagulant effects of mulga snake venom. Tests performed with these reagents should be interpreted with caution. Copyright © 2014 Royal College of pathologists of Australasia.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Clinical sciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Prasad, R (Dr Ritam Prasad)
ID Code:120057
Year Published:2014
Web of Science® Times Cited:5
Deposited By:Medicine
Deposited On:2017-08-09
Last Modified:2017-08-09

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