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Wall-less stationary pH boundary for stacking proteins on a glass microchip


See, HH and Guijt, RM and Breadmore, MC, Wall-less stationary pH boundary for stacking proteins on a glass microchip, Proceedings of MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 25-29 October, Gyeongyu, Korea, pp. 1969-1971. ISBN 978-0-9798064-8-3 (2015) [Refereed Conference Paper]

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Copyright 2015 Chemical and Biological Microsystems Society (CBMS)

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This work reports a new electrophoretic stacking protocol based on the formation of a stationary pH boundary to concentrate and analyze proteins on a glass microchip. The feasibility of continuously loading proteins from a continuously flowing sample to increase the amount of analyte injected prior to separation and detection was demonstrated. An increase in protein concentration of about 800-1000 fold was achieved in approximately 5 min of stacking, and the subsequent separation and detection of three different protein bands namely BSA, β-lactoglobulin and as r-phycoerthrin were achieved within 3 min.

Item Details

Item Type:Refereed Conference Paper
Keywords:protein preconcentration, stacking, pH boundary
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Electroanalytical chemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:See, HH (Dr Hong Heng See)
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
UTAS Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:119997
Year Published:2015
Deposited By:Chemistry
Deposited On:2017-08-08
Last Modified:2018-03-08

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