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Wall-less stationary pH boundary for stacking proteins on a glass microchip
Citation
See, HH and Guijt, RM and Breadmore, MC, Wall-less stationary pH boundary for stacking proteins on a glass microchip, Proceedings of MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 25-29 October, Gyeongyu, Korea, pp. 1969-1971. ISBN 978-0-9798064-8-3 (2015) [Refereed Conference Paper]
Copyright Statement
Copyright 2015 Chemical and Biological Microsystems Society (CBMS)
Official URL: http://mtas.hdasan.com/
Abstract
This work reports a new electrophoretic stacking protocol based on the formation of a stationary pH boundary to concentrate and analyze proteins on a glass microchip. The feasibility of continuously loading proteins from a continuously flowing sample to increase the amount of analyte injected prior to separation and detection was demonstrated. An increase in protein concentration of about 800-1000 fold was achieved in approximately 5 min of stacking, and the subsequent separation and detection of three different protein bands namely BSA, β-lactoglobulin and as r-phycoerthrin were achieved within 3 min.
Item Details
| Item Type: | Refereed Conference Paper |
|---|---|
| Keywords: | protein preconcentration, stacking, pH boundary |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Electroanalytical Chemistry |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| Author: | See, HH (Dr Hong Heng See) |
| Author: | Guijt, RM (Dr Rosanne Guijt) |
| Author: | Breadmore, MC (Professor Michael Breadmore) |
| ID Code: | 119997 |
| Year Published: | 2015 |
| Deposited By: | Chemistry |
| Deposited On: | 2017-08-08 |
| Last Modified: | 2018-03-08 |
| Downloads: | 0 |
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