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Nanofluidic trap for DNA extraction from biological samples
Citation
Shallan, AI and Guijt, RM and Breadmore, MC, Nanofluidic trap for DNA extraction from biological samples, Proceedings of MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 25-29 October, Gyeongyu, Korea, pp. 1963-1965. ISBN 978-097980648-3 (2015) [Refereed Conference Paper]
Copyright Statement
Copyright 2015 Chemical and Biological Microsystems Society (CBMS)
Official URL: http://mtas.hdasan.com/
Abstract
A new device seamlessly integrating various electrokinetic processes enables the analysis of short ssDNA (20 bases) directly from whole blood. An electrokinetic trap was integrated to achieve extraction, concentration, and desalting of ssDNA in 200 s followed by a 90 s electrophoretic separation. The trap consists of two sequentially coupled nanojunctions with different pore size and exhibits selective ion transport properties. Depletion forces near the nanojunctions and the apparent electromigration were tuned to enable the trapping and subsequent analysis of fluorescein labeled-ssDNA. The quantitation limit (S/N ratio = 10) was 12.5 nM using 40 μL of spiked whole blood.
Item Details
| Item Type: | Refereed Conference Paper |
|---|---|
| Keywords: | sample preparation, controlled dielectric breakdown, electrokinetic trapping, ssDNA |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Electroanalytical Chemistry |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| Author: | Shallan, AI (Ms Aliaa Shallan) |
| Author: | Guijt, RM (Dr Rosanne Guijt) |
| Author: | Breadmore, MC (Professor Michael Breadmore) |
| ID Code: | 119996 |
| Year Published: | 2015 |
| Deposited By: | Chemistry |
| Deposited On: | 2017-08-08 |
| Last Modified: | 2017-10-05 |
| Downloads: | 0 |
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