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Nanofluidic trap for DNA extraction from biological samples

Citation

Shallan, AI and Guijt, RM and Breadmore, MC, Nanofluidic trap for DNA extraction from biological samples, Proceedings of MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 25-29 October, Gyeongyu, Korea, pp. 1963-1965. ISBN 978-097980648-3 (2015) [Refereed Conference Paper]

Copyright Statement

Copyright 2015 Chemical and Biological Microsystems Society (CBMS)

Official URL: http://mtas.hdasan.com/

Abstract

A new device seamlessly integrating various electrokinetic processes enables the analysis of short ssDNA (20 bases) directly from whole blood. An electrokinetic trap was integrated to achieve extraction, concentration, and desalting of ssDNA in 200 s followed by a 90 s electrophoretic separation. The trap consists of two sequentially coupled nanojunctions with different pore size and exhibits selective ion transport properties. Depletion forces near the nanojunctions and the apparent electromigration were tuned to enable the trapping and subsequent analysis of fluorescein labeled-ssDNA. The quantitation limit (S/N ratio = 10) was 12.5 nM using 40 μL of spiked whole blood.

Item Details

Item Type:Refereed Conference Paper
Keywords:sample preparation, controlled dielectric breakdown, electrokinetic trapping, ssDNA
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Electroanalytical Chemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Shallan, AI (Ms Aliaa Shallan)
Author:Guijt, RM (Dr Rosanne Guijt)
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:119996
Year Published:2015
Deposited By:Chemistry
Deposited On:2017-08-08
Last Modified:2017-10-05
Downloads:0

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