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Ground-truthing phylotype assignments for Antarctic invertebrates

Citation

Czechowski, P and Clarke, L and Cooper, A and Stevens, M, Ground-truthing phylotype assignments for Antarctic invertebrates, DNA Barcodes, 5, (1) pp. 1-13. ISSN 2299-1077 (2017) [Refereed Article]


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Copyright Statement

Copyright 2017 Paul Czechowski, et al. Licensed under Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) https://creativecommons.org/licenses/by-nc-nd/3.0/

DOI: doi:10.1515/dna-2017-0001

Abstract

Biodiversity information from Antarctic terrestrial habitats helps conservation efforts, but the distribution and diversity particularly of microinvertebrates remains poorly understood. Springtails, mites, tardigrades, nematodes and rotifers are difficult to identify using morphological features, hence DNA-based metabarcoding methods are well suited for their study. We compared taxonomy assignments of a high throughput sequencing metabarcoding approach using one ribosomal DNA (18S rDNA) and one mitochondrial DNA (cytochrome c oxidase subunit I - COI) marker with morphological reference data. Specifically, we compared metabarcoding or morphological taxonomic assignments on multiple taxonomic levels in an artificial DNA blend containing Australian invertebrates, and in seven extracts of Antarctic soils containing known micro-faunal taxa. Avoiding arbitrary application of metabarcoding analysis parameters, we calibrated those parameters with metabarcoding data from non-Antarctic soils. Metabarcoding approaches employing 18S rDNA and COI markers enabled detection of small and cryptic Antarctic invertebrates, and on low taxonomic ranks 18S data outperformed COI data in this respect. Morphological taxonomy determination did not outperform metabarcoding approaches. Our study demonstrates how barcoding markers can be tested prior to their application to specific taxonomic groups, and that taxonomy fidelity of markers needs to be validated in relation to environment, taxa, and available reference information.

Item Details

Item Type:Refereed Article
Keywords:environmental DNA, metataxonomic, mitochondrial cytochrome c oxidase I, COI, 18S rDNA, Illumina, 454, biodiversity survey
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Antarctic and Sub-Antarctic Flora, Fauna and Biodiversity
Author:Clarke, L (Dr Laurence Clarke)
ID Code:119953
Year Published:2017
Deposited By:CRC-Antarctic Climate & Ecosystems
Deposited On:2017-08-08
Last Modified:2017-09-06
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