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Recruiting a new substrate for triacylglycerol synthesis in plants: the monoacylglycerol acyltransferase pathway

Citation

Petrie, JR and Vanhercke, T and Shrestha, P and El Tahchy, A and White, A and Zhou, X-R and Liu, Q and Mansour, MP and Nichols, PD and Singh, SP, Recruiting a new substrate for triacylglycerol synthesis in plants: the monoacylglycerol acyltransferase pathway, PLoS One, 7, (4) Article e35214. ISSN 1932-6203 (2012) [Refereed Article]


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Copyright Statement

Copyright 2012 Petrie et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pone.0035214

Abstract

Background

Monoacylglycerol acyltransferases (MGATs) are predominantly associated with lipid absorption and resynthesis in the animal intestine where they catalyse the first step in the monoacylglycerol (MAG) pathway by acylating MAG to form diacylglycerol (DAG). Typical plant triacylglycerol (TAG) biosynthesis routes such as the Kennedy pathway do not include an MGAT step. Rather, DAG and TAG are synthesised de novo from glycerol-3-phosphate (G-3-P) by a series of three subsequent acylation reactions although a complex interplay with membrane lipids exists.

Methodology/Principal Findings

We demonstrate that heterologous expression of a mouse MGAT acyltransferase in Nicotiana benthamiana significantly increases TAG accumulation in vegetative tissues despite the low levels of endogenous MAG substrate available. In addition, DAG produced by this acyltransferase can serve as a substrate for both native and coexpressed diacylglycerol acyltransferases (DGAT). Finally, we show that the Arabidopsis thaliana GPAT4 acyltransferase can produce MAG in Saccharomyces cerevisiae using oleoyl-CoA as the acyl-donor.

Conclusions/Significance

This study demonstrates the concept of a new method of increasing oil content in vegetative tissues by using MAG as a substrate for TAG biosynthesis. Based on in vitro yeast assays and expression results in N. benthamiana, we propose that co-expression of a MAG synthesising enzyme such as A. thaliana GPAT4 and a MGAT or bifunctional M/DGAT can result in DAG and TAG synthesis from G-3-P via a route that is independent and complementary to the endogenous Kennedy pathway and other TAG synthesis routes.

Item Details

Item Type:Refereed Article
Keywords:triacylglycerol, MGATs, TAG
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Analytical Biochemistry
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Nichols, PD (Dr Peter Nichols)
ID Code:119643
Year Published:2012
Web of Science® Times Cited:42
Deposited By:Centre for Ecology and Biodiversity
Deposited On:2017-08-03
Last Modified:2017-09-20
Downloads:5 View Download Statistics

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